Related Experiment Video
Updated: Oct 2, 2026
Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling
Published on: March 4, 2018
Effects of Sucrose and Trehalose Supplementation on Zebrafish (Danio rerio) Sperm Quality During Cold Storage
Blenda R N Vilela1,2, Pilar Vallejo-Soto1, Carlos Montero-Palma1
1Veterinary Reproduction Group (AGR-275), Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universidad de Córdoba, Córdoba, Spain.
Abstract:
This study evaluated the effect of disaccharide supplementation on zebrafish sperm stored at 5°C for 24 h. Seven pooled replicates from 60 broodfish were used. Semen samples were initially cooled and, after 1 h, supplemented with either HBSS (E-C), Sucrose 0.13M (E-S13), Trehalose 0.13M (E-T13) or Trehalose 0.26M (E-T26). Sperm motility (computer-assisted sperm analysis) and plasma membrane integrity (flow cytometry) were evaluated over 24 h of cool storage. During the first 2 h, E-T26 decreased sperm motility values. After 24 h, E-S13 and E-T13 showed higher motility than E-C and E-T26. In conclusion, sucrose and trehalose supplementation improved sperm motility after 24 h of cold storage. Further studies are needed to assess the effect of disaccharide supplementation after shorter storage periods.
