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The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins”   is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...
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The epidermis is made of four or five layers of epithelial cells, depending on its location in the body. From deep to superficial, these layers are the stratum basale, stratum spinosum, stratum granulosum, stratum lucidum, and stratum corneum.
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The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
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Live Imaging of Cell Extrusion from the Epidermis of Developing Zebrafish
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E-cadherin expression pattern during zebrafish embryonic epidermis development.

María Florencia Sampedro1,2, María Fernanda Izaguirre1, Valeria Sigot1,2

  • 1Laboratorio de Microscopía Aplicada a Estudios Moleculares y Celulares (LAMAE), Facultad de Ingeniería, Universidad Nacional de Entre Ríos, Oro Verde, 3100, Argentina.

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|March 9, 2019
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Summary
This summary is machine-generated.

E-cadherin levels increase during zebrafish embryonic skin development, driving cell shape changes and hexagonal packing. This study quantifies E-cadherin

Keywords:
3D segmentationKey words: E-cadherin; adherens junctions; zebrafish epidermis; enveloping layer (EVL)deconvolutionepidermis basal layer (EBL)

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Related Experiment Videos

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Biophysics

Background:

  • E-cadherin is a key adhesion receptor in epithelial adherens junctions (AJs).
  • E-cadherin regulates cell shape, size, and packing density to maintain epidermal tissue integrity.
  • Its role in zebrafish embryonic epidermis development is not well understood.

Purpose of the Study:

  • To quantitatively assess the spatial and temporal distribution of E-cadherin in zebrafish embryonic epidermis.
  • To correlate E-cadherin levels with changes in cell morphology and tissue organization.

Main Methods:

  • Utilized immunofluorescence, deconvolution microscopy, and 3D segmentation of adherens junctions (AJs).
  • Quantified E-cadherin distribution in zebrafish epidermis between 24 and 72 hours post-fertilization (hpf).

Main Results:

  • E-cadherin protein levels increased significantly between 31 and 48 hpf.
  • Higher E-cadherin correlated with increased cell density and hexagonal cell shapes in the enveloping layer (EVL).
  • New cell-cell contacts were established in the epidermal basal layer (EBL).

Conclusions:

  • Increasing E-cadherin levels are associated with significant cell shape modulation towards hexagonal packing.
  • This process is crucial for epidermal morphogenesis during zebrafish embryonic development.