Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Standard Enthalpy of Formation02:37

Standard Enthalpy of Formation

49.2K
Enthalpy changes are typically tabulated for reactions in which both the reactants and products are at the same conditions. A standard state is a commonly accepted set of conditions used as a reference point for the determination of properties under other different conditions. For chemists, the IUPAC standard state refers to materials under a pressure of 1 bar and solutions at 1 M and does not specify a temperature. Many thermochemical tables list values with a standard state of 1 atm. Because...
49.2K
Calculating Standard Free Energy Changes02:49

Calculating Standard Free Energy Changes

24.9K
The free energy change for a reaction that occurs under the standard conditions of 1 bar pressure and at 298 K is called the standard free energy change. Since free energy is a state function, its value depends only on the conditions of the initial and final states of the system. A convenient and common approach to the calculation of free energy changes for physical and chemical reactions is by use of widely available compilations of standard state thermodynamic data. One method involves the...
24.9K
Theory of Attribution I: Correspondent Inference Theory01:15

Theory of Attribution I: Correspondent Inference Theory

562
Correspondent inference theory, proposed by Jones and Davis in 1965, seeks to explain how individuals infer stable personality traits from observed behaviors. It suggests that people attribute actions to underlying dispositions rather than external circumstances, particularly when the behavior appears intentional and socially significant.Voluntary Behavior and Dispositional AttributionAccording to this theory, individuals are more likely to attribute behavior to personal traits when it appears...
562
Standard Entropy Change for a Reaction03:00

Standard Entropy Change for a Reaction

24.5K
Entropy is a state function, so the standard entropy change for a chemical reaction (ΔS°rxn) can be calculated from the difference in standard entropy between the products and the reactants.
24.5K
Standard Electrode Potentials03:02

Standard Electrode Potentials

50.3K
On comparing the reactivity of silver and lead, it is observed that the two ionic species, Ag+ (aq) and Pb2+ (aq), show a difference in their redox reactivity towards copper: the silver ion undergoes spontaneous reduction, while the lead ion does not. This relative redox activity can be easily quantified in electrochemical cells by a property called cell potential. This property is commonly known as cell voltage in electrochemistry, and it is a measure of the energy which accompanies the charge...
50.3K
Measurement: Standard Units03:38

Measurement: Standard Units

79.8K
Every measurement provides three kinds of information: the size or magnitude of the measurement (a number), a standard of comparison for the measurement (a unit), and an indication of the uncertainty of the measurement. While the number and unit are explicitly represented when a quantity is written, the uncertainty is an aspect of the errors in the measurement results.
79.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessing the contribution of rare variants to congenital heart disease through a large-scale case-control exome study.

NPJ genomic medicine·2026
Same author

Phosphorylated toll-like receptor 4 defines a high-risk sepsis endotype.

Critical care (London, England)·2026
Same author

AI-Supported, Integrative Prediction of Postoperative Delirium: Protocol for the CONFUSED Study.

JMIR research protocols·2026
Same author

Schizophrenia risk gene ZNF804A controls ribosome localization and synaptogenesis in developing human neurons.

Science advances·2026
Same author

An AI-Ready Phosphorylation Meta-Analysis for <i>Saccharomyces cerevisiae</i>.

Journal of proteome research·2026
Same author

Blood proteomics: insights from public data.

Genome biology·2026

Related Experiment Video

Updated: Feb 2, 2026

Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection
10:02

Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection

Published on: December 19, 2025

450

Protein Inference Using PIA Workflows and PSI Standard File Formats.

Julian Uszkoreit1, Yasset Perez-Riverol2, Britta Eggers1

  • 1Ruhr University Bochum , Medical Faculty, Medizinisches Proteom-Center , Universitaetsstrasse 150 , D-44801 Bochum , Germany.

Journal of Proteome Research
|November 27, 2018
PubMed
Summary

Protein inference is crucial in proteomics for identifying proteins from peptide data. This study introduces PIA, a tool enhancing protein inference and supporting PSI standards, with new features for KNIME workflow integration.

Keywords:
computational proteomicsprotein inferenceprotein isoformsspectrum identificationstandard formatsworkflows

More Related Videos

A Quantitative Fitness Analysis Workflow
11:39

A Quantitative Fitness Analysis Workflow

Published on: August 13, 2012

14.9K
Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
10:33

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

Published on: January 8, 2014

7.6K

Related Experiment Videos

Last Updated: Feb 2, 2026

Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection
10:02

Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection

Published on: December 19, 2025

450
A Quantitative Fitness Analysis Workflow
11:39

A Quantitative Fitness Analysis Workflow

Published on: August 13, 2012

14.9K
Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
10:33

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

Published on: January 8, 2014

7.6K

Area of Science:

  • Proteomics
  • Bioinformatics
  • Computational Biology

Background:

  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a primary high-throughput proteomics method.
  • Limitations in mass spectrometry instruments necessitate shotgun proteomics, focusing on peptide identification.
  • Protein inference, inferring proteins from identified peptides, is a critical but often overlooked step.

Purpose of the Study:

  • To present the features of the PIA (Protein Inference Algorithms) tool for protein inference.
  • To emphasize the importance of PSI standard file formats in proteomics data analysis.
  • To showcase PIA's integration with workflow environments like the KNIME Analytics Platform.

Main Methods:

  • Utilized LC-MS/MS data for peptide identification.
  • Employed the PIA software tool for protein inference.
  • Integrated PIA with the KNIME Analytics Platform for workflow analysis.
  • Benchmarked PIA performance using a published isoform detection dataset.

Main Results:

  • PIA offers enhanced features for protein inference from peptide data.
  • PIA is the sole software supporting all current PSI standards for spectrum identification and protein inference.
  • New PIA nodes for KNIME Analytics Platform facilitate workflow integration.
  • PIA demonstrates effective performance in isoform detection benchmarking.

Conclusions:

  • PIA is a valuable open-source tool for improving protein inference in proteomics.
  • Adherence to PSI standards and integration with workflow environments enhance proteomics data analysis.
  • PIA provides a robust solution for complex protein inference challenges, including isoform detection.