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Related Experiment Video

Updated: Feb 2, 2026

Avidity-based Extracellular Interaction Screening AVEXIS for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions

Avidity-based Extracellular Interaction Screening AVEXIS for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions

Published on: March 5, 2012

Pooled extracellular receptor-ligand interaction screening using CRISPR activation.

Zheng-Shan Chong¹, Shuhei Ohnishi², Kosuke Yusa²

¹Cell Surface Signalling Laboratory, Wellcome Sanger Institute, Cambridge, CB10 1SA, UK.

|November 28, 2018

Summary

This summary is machine-generated.

Identifying cell surface receptors and their ligands is challenging. This study introduces a CRISPR activation method to discover these interactions, successfully identifying myelin-associated inhibitory proteins as ligands for ADGRB1.

Keywords:

CRISPR activation Cell signaling Cell surface receptors Extracellular protein interactions Flow cytometry G-protein-coupled receptor Genome-wide screening Monoclonal antibodies

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Avidity-based Extracellular Interaction Screening AVEXIS for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions

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Published on: March 5, 2012

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

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Published on: September 4, 2019

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Area of Science:

Cell Biology
Molecular Biology
Genomics

Background:

Extracellular interactions involving cell surface receptors are crucial for cellular signaling and adhesion.
Identifying specific receptor-ligand pairs remains a significant technical hurdle in biological research.

Purpose of the Study:

To develop and validate a genome-wide, cell-based CRISPR activation screening method for identifying receptors of defined ligands.
To apply this novel technique to discover ligands for adhesion G-protein-coupled receptors (GPCRs).

Main Methods:

Utilized CRISPR activation (CRISPRa) screening in a cell-based system.
Employed pooled and individual binding probe strategies to identify receptor-ligand interactions.
Applied the method to investigate ligands for adhesion GPCRs.

Main Results:

Demonstrated the ability to unambiguously identify receptors for both high-affinity antibodies and low-affinity ligands.
Successfully identified Nogo myelin-associated inhibitory proteins as ligands for the adhesion G-protein-coupled receptor B1 (ADGRB1).

Conclusions:

The developed CRISPRa-based method provides a powerful tool for large-scale identification of extracellular receptor-ligand pairs.
This approach significantly advances the ability to map cell surface receptor-ligand interactions across the genome.