Related Experiment Video
Updated: Feb 2, 2026

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
Published on: May 4, 2014
Faster, sharper, and deeper: structured illumination microscopy for biological imaging
1Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA. yicong.wu@nih.gov.
Abstract:
Structured illumination microscopy (SIM) allows rapid, super-resolution (SR) imaging in live specimens. We review recent technical advances in SR-SIM, with emphasis on imaging speed, resolution, and depth. Since its introduction decades ago, the technique has grown to offer myriad implementations, each with its own strengths and weaknesses. We discuss these, aiming to provide a practical guide for biologists and to highlight which approach is best suited to a given application.
Related Concept Videos
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
What is Conservation Biology?
Biological Effects of Radiation
Structure of Lipids
Synthetic Biology
Golden rice
Golden rice is a genetically modified...
Biological Causes of Schizophrenia
Genetic Factors in Schizophrenia
The genetic basis of schizophrenia is strongly supported by family and twin...

