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Nonlytic Recombinant Phage Tail Fiber Protein for Specific Recognition of Pseudomonas aeruginosa

Yong He1,2, Yanli Shi1, Mengli Liu1

  • 1Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Ministry of Education), College of Pharmaceutical Sciences , Southwest University , Chongqing 400716 , China.

Analytical Chemistry
|November 28, 2018
PubMed

Insights

A novel recombinant protein (P069) from Pseudomonas aeruginosa phage enables sensitive and specific bacterial detection. This tool offers rapid identification of P. aeruginosa in complex samples, aiding infectious disease diagnosis.

Area of Science:

  • Microbiology and Biotechnology
  • Bacterial Detection and Diagnostics

Background:

  • Rapid and accurate bacterial detection is essential for diagnosing infectious diseases.
  • Pseudomonas aeruginosa (P. aeruginosa) poses significant health risks, necessitating reliable detection methods.

Purpose of the Study:

  • To develop a novel biorecognition element for the specific detection of P. aeruginosa.
  • To evaluate the efficacy of a recombinant tail fiber protein (P069) for bacterial capture and detection.

Main Methods:

  • Expression and renaturation of recombinant P069 protein from P. aeruginosa phage in E. coli.
  • Utilizing P069 for bacterial cell capture without lytic activity.
  • Employing bioluminescent and fluorescent methods for P. aeruginosa detection.

Main Results:

  • Recombinant P069 protein was successfully produced, renatured, and retained P. aeruginosa recognition capabilities.
  • Detection limits achieved were 6.7 × 10^2 CFU mL^-1 (bioluminescent) and 1.7 × 10^2 CFU mL^-1 (fluorescent).
  • P069 demonstrated species-specific detection, avoiding false negatives, with successful application in spiked samples (88-98% recovery).

Conclusions:

  • Recombinant P069 is an effective and specific biorecognition element for P. aeruginosa detection.
  • P069 facilitates flexible bacterial cell capture for downstream analysis in complex matrices.
  • This method offers a promising approach for rapid and accurate P. aeruginosa diagnostics.

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