Modeling myeloid malignancies with patient-derived iPSCs
1Department of Oncological Sciences, Department of Medicine, Tisch Cancer Institute, and Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Experimental Hematology
|November 28, 2018
Summary
Patient-derived induced pluripotent stem cells (iPSCs) are increasingly used to model myeloid malignancies. This approach helps understand cancer development and offers new avenues for hematology research.
Area of Science:
- Hematology
- Stem Cell Biology
- Oncology
Background:
- Patient-derived induced pluripotent stem cells (iPSCs) offer powerful models for human diseases, especially in hematology.
- Modeling malignant hematologic disorders with iPSCs has lagged behind monogenic diseases due to derivation and standardization challenges.
Purpose of the Study:
- To review the progress and challenges in modeling myeloid malignancies using patient-derived iPSCs.
- To highlight the utility of iPSCs in understanding clonal evolution and the cell-of-origin in leukemia.
Main Methods:
- Derivation of iPSCs from cancer cells and healthy controls.
- Development of robust hematopoietic differentiation protocols.
- Standardization of phenotypic and molecular assays for iPSC-derived hematopoietic cells.
Main Results:
- Recent iPSC models of myeloid malignancies leverage clonal heterogeneity for comparative studies.
- Comparisons reveal the significance of cell-of-origin in oncogenesis and gene-cancer interactions.
- iPSC-derived hematopoiesis models recapitulate aspects of disease phenotypes and molecular signatures.
Conclusions:
- iPSC modeling is crucial for investigating oncogenesis in myeloid malignancies.
- Advancements in iPSC derivation and scalable differentiation protocols are needed for wider adoption.
- The field is poised for increased use of iPSC models in hematology research.
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