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Updated: Feb 2, 2026

High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test
Mohamed Hamidouche1, Nassira Belmessabih1, Abderrahmane Boubguira1
1Laboratory of production and development of viral veterinary vaccines, Institut Pasteur of Algeria. Annex of Kouba, 01 rue de caire, kouba, Algiers, Algeria.
Abstract:
Virus neutralisation test (VNT) of capripoxvirus (CaPVs) was studied to assess the post-vaccination (vaccine effectiveness) or post-infection antibodies level using two methods: alpha-VNT and beta-VNT which are generally carried out to measure the Neutralising Index (N.I.) and the serum Antibody titre (TAb) respectively. The authors have demonstrated that a positive correlation exists between N.I. and TAb values, this study aimed to add more evidence to this correlation by establishing a graph and its mathematical equations. We found that: N.I. = (1.489 Log TAb) + 1.331; this serves as a base to calculate N.I. using TAb values measured by beta-VNT without going through alpha-VNT and vice versa. At the end of this study, we evaluated the equation accuracy by two parameters; the deviation (d) and the error percentage, which were d = 0.2 and error (%) = 8%, respectively.
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