Related Experiment Video

Updated: Feb 2, 2026

Establishment of a Surgically-induced Model in Mice to Investigate the Protective Role of Progranulin in Osteoarthritis
07:58

Establishment of a Surgically-induced Model in Mice to Investigate the Protective Role of Progranulin in Osteoarthritis

Published on: February 25, 2014

29.8K

Deficiency of the pattern-recognition receptor CD14 protects against joint pathology and functional decline in a

Nisha Sambamurthy1,2, Cheng Zhou1,2, Vu Nguyen1,2

  • 1Translational Musculoskeletal Research Center, Corporal Michael J. Crescenz Department of Veterans Affairs Medical Center, Philadelphia, Pennsylvania, United States of America.

Plos One
|November 29, 2018
PubMed
Abstract

Insights

CD14 deficiency protected mice from osteoarthritis progression and joint pain in a murine model. This suggests CD14 is a potential therapeutic target for treating osteoarthritis.

Area of Science:

  • Immunology
  • Rheumatology
  • Orthopedics

Background:

  • CD14 is a pattern-recognition receptor on monocytes/macrophages involved in innate inflammatory signaling.
  • Elevated soluble CD14 in synovial fluid correlates with osteoarthritis (OA) symptoms and disease progression.

Purpose of the Study:

  • To investigate the role of the CD14 receptor in the development and progression of osteoarthritis (OA).
  • To evaluate the therapeutic potential of targeting CD14 in OA using a murine model.

Main Methods:

  • Destabilization of the medial meniscus (DMM) surgery was performed on wild-type (WT) and CD14-deficient (CD14-/-) mice to induce OA.
  • Histopathology and microCT were used to assess cartilage damage and subchondral bone remodeling.
  • Macrophage markers were analyzed via flow cytometry and ddPCR; locomotive activity was monitored to evaluate joint pain.

Main Results:

  • CD14-/- mice showed significantly less cartilage damage at 19 weeks post-DMM compared to WT mice.
  • CD14 deficiency protected against subchondral bone changes and preserved locomotive activity after DMM.
  • Absence of CD14 led to reduced expression of CCR7 (M1 macrophage marker) but did not alter CD68 or CD163 expression.

Conclusions:

  • CD14 plays a significant role in the progression of structural and functional aspects of osteoarthritis.
  • Targeting CD14 may offer a novel therapeutic strategy for managing osteoarthritis.

More Related Videos

Systematic Assessment of Mammalian Skull Specimens for Dental and Temporomandibular Joint Pathology07:26

Systematic Assessment of Mammalian Skull Specimens for Dental and Temporomandibular Joint Pathology

Published on: August 22, 2022

2.0K
Anterior Cruciate Ligament Transection and Synovial Fluid Lavage in a Rodent Model to Study Joint Inflammation and Posttraumatic Osteoarthritis06:28

Anterior Cruciate Ligament Transection and Synovial Fluid Lavage in a Rodent Model to Study Joint Inflammation and Posttraumatic Osteoarthritis

Published on: September 2, 2025

1.4K

Related Concept Videos

Functional Classification of Joints01:09

Functional Classification of Joints

Functional Classification of Joints
The functional classification of joints is determined by the amount of mobility between the adjacent bones. Joints are functionally classified as a synarthrosis or immobile joint, an amphiarthrosis or slightly moveable joint, or as a diarthrosis, a freely moveable joint. Fibrous and cartilaginous joints can be functionally classified as either synarthroses  or amphiarthroses, whereas all synovial joints are classified as diarthroses.
Synarthrosis
An...
6.8K
Magnetic Declination01:19

Magnetic Declination

Magnetic declination is the angle between true north, which aligns with the Earth's rotational axis, and magnetic north, which follows the direction of the Earth's magnetic field. This discrepancy exists because the magnetic poles do not coincide with the geographic poles. The value of magnetic declination depends on the observer's location on Earth and is subject to changes over time due to the dynamic nature of the Earth's magnetic field.The declination is called eastern when magnetic north...
453
Conservation of Declining Populations02:07

Conservation of Declining Populations

Conservation of declining population focuses on ways of detecting, diagnosing, and halting a population decline. The approach uses methods to prevent populations from going extinct.
13.3K
The Two-State Receptor Model01:29

The Two-State Receptor Model

The two-state receptor model explains a drug's interaction with receptors, such as G protein-coupled receptors and ligand-gated ion channels, to induce or inhibit a biological response. When no natural ligands are present, a receptor exists in an equilibrium of inactive (Ri) and active (Ra) conformations. The inactive form does not produce a response, while the active form generates a basal effect known as constitutive activity.
The binding affinity of a drug determines its interaction with...
3.1K
Structural Joints: Synovial Joints01:16

Structural Joints: Synovial Joints

Synovial joints are the most common type of joint in the body. A key structural characteristic for a synovial joint is the presence of a joint cavity. This fluid-filled space is where the articulating surfaces of the bones contact each other. Also, unlike fibrous or cartilaginous joints, the articulating bone surfaces at a synovial joint are not directly connected to each other with fibrous connective tissue or cartilage. This gives the bones of a synovial joint the ability to move smoothly...
6.8K
Structural Joints: Fibrous Joints01:03

Structural Joints: Fibrous Joints

Fibrous joints are a type of joint where the bones are connected by fibrous connective tissue. These joints provide stability and minimal to no movement between the articulating bones. There are three types of fibrous joints.
Suture
All the bones of the skull, except for the mandible, are joined to each other by a fibrous joint called a suture. The fibrous connective tissue found at a suture strongly unites the adjacent skull bones and thus helps to protect the brain and form the face. In...
3.8K