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Related Concept Videos

RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
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Alternative RNA Splicing02:18

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Proteins: From Genes to Degradation02:11

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Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
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Mass Spectrometry: Complex Analysis01:21

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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What is Gene Expression?01:42

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Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
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Faustovirus E12 Transcriptome Analysis Reveals Complex Splicing in Capsid Gene.

Amina Cherif Louazani1, Emeline Baptiste1, Anthony Levasseur1

  • 1Assistance Publique - Hôpitaux de Marseille (AP-HM), Microbes, Evolution, Phylogeny and Infection (MEΦI), Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Institut de Recherche pour le Développement IRD 198, Aix-Marseille Université UM63, Marseille, France.

Frontiers in Microbiology
|November 30, 2018
PubMed
Summary
This summary is machine-generated.

Faustovirus E12 gene expression reveals a large major capsid protein (MCP) transcript with 13 exons. This giant virus utilizes both self-splicing and spliceosome-like introns, with non-canonical splice sites.

Keywords:
capsidfaustovirusgiant virussplicingtranscriptome

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Area of Science:

  • Virology
  • Molecular Biology
  • Genomics

Background:

  • Faustoviruses are giant viruses infecting amoebae, distantly related to African swine fever virus.
  • The major capsid protein (MCP) of Faustovirus E12 is encoded by a sequence scattered across a large genomic region.

Purpose of the Study:

  • To investigate the gene expression and splicing of Faustovirus E12 during its replicative cycle.
  • To characterize the transcript encoding the major capsid protein (MCP).

Main Methods:

  • RNA sequencing (RNA-seq) at nine time points.
  • Paired-end sequencing on MiSeq instrument.
  • Double-round spliced alignment to identify splice junctions.

Main Results:

  • Identified 26 different splice junctions, with junction reads comprising 2% of mapped reads.
  • Described a 1,939 bp transcript for the MCP, composed of 13 exons.
  • Detected two intron types: 5 self-splicing group I introns and 7 spliceosome-like introns with non-canonical splice sites.

Conclusions:

  • Faustovirus E12 gene expression involves complex splicing mechanisms.
  • The MCP gene contains a large transcript with numerous introns, including non-canonical splice sites.
  • This study provides insights into the molecular mechanisms of giant virus gene expression.