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Microchip Electrophoresis Containing Electrodes for Integrated Electrochemical Detection
Lucas Paines Bressan1, Dosil Pereira de Jesus1, Dulan Bandara Gunasekara2
1Chemistry Institute, State University of Campinas, Campinas, SP, Brazil.
Methods in Molecular Biology (Clifton, N.J.)
|November 30, 2018
Summary
This study details preparing platinum band electrodes on glass for polydimethylsiloxane (PDMS) microchips. These microchips enable efficient separation and electrochemical detection of nitrite ions.
Area of Science:
- Analytical Chemistry
- Electrochemistry
- Microfluidics
Background:
- Microchip electrophoresis offers versatile separation capabilities.
- Electrochemical detection is advantageous for microdevices due to ease of fabrication, sensitivity, and selectivity.
- Integrating electrochemical detection with microchip electrophoresis enhances analytical performance.
Purpose of the Study:
- To describe the preparation of platinum band electrodes on glass substrates.
- To couple these electrodes with polydimethylsiloxane (PDMS) microchips.
- To achieve the separation and electrochemical detection of nitrite.
Main Methods:
- Fabrication of platinum band electrodes on glass.
- Integration of electrodes with polydimethylsiloxane (PDMS) microchips.
- Utilizing an isolated potentiostat for electrochemical detection.
Main Results:
- Successful preparation and integration of Pt band electrodes on glass with PDMS microchips.
- Demonstrated capability for separation of analytes within the microchip.
- Achieved sensitive and selective electrochemical detection of nitrite.
Conclusions:
- The developed microchip electrophoresis system with Pt electrodes is effective for nitrite separation and detection.
- This approach offers a promising platform for sensitive electrochemical analysis in microdevices.
- The integration of electrochemical detection with microfluidic devices is feasible and advantageous.
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