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Updated: Feb 1, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
Published on: October 24, 2018
Rapid method for simultaneous determination of ascorbic acid and zinc in effervescent tablets by capillary zone
Michelle Miranda A C Ribeiro1, Aliceana A Prado1, Alex Domingues Batista1
1Institute of Chemistry, Federal University of Uberlandia, Uberlandia, MG, Brazil.
Abstract:
Ascorbic acid and zinc are essential nutrients that play important roles in nutrition, immune support, and maintenance of health. For this reason, both compounds are widely used as ingredients in dietary supplements. We report, for the first time, an analytical method for fast simultaneous determination of ascorbic acid and zinc. A single analysis run is possible every 80 s (45 injections/h). The method is based on capillary electrophoresis with capacitively coupled contactless conductivity detection using a fused silica capillary with 50 cm length (effective length of 10 cm). The separation was achieved by using a background electrolyte composed by 30 mmol/L of 2-(morpholin-4-yl)ethane-1-sulfonic acid and 30 mmol/L of histidine, pH 6.1. The detection limits were 10 and 20 μmol/L and recovery values for spiked samples were 101 and 100% for zinc and ascorbic acid, respectively. The results obtained with the developed procedure were compared to those obtained by titration (ascorbic acid) and flame atomic absorption spectroscopy (zinc), and no statistically significant differences were observed (95% confidence level).
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