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A cDNA clone encoding a 10.8 kDa photosystem I polypeptide of barley
J S Okkels1, L B Jepsen, L S Hønberg
1Department of Plant Physiology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.
FEBS Letters
|September 12, 1988
Abstract:
A cDNA clone encoding the barley photosystem I polypeptide which migrates with an apparent molecular mass of 16 kDa on SDS-polyacrylamide gels has been isolated. The 634 bp sequence of this clone has been determined and contains one large open reading frame coding for a 15,457 Da precursor polypeptide. The molecular mass of the mature polypeptide is 10,821 Da. The amino acid sequence of the transit peptide indicates that the polypeptide is routed towards the stroma side of the thylakoid membrane. The hydropathy plot of the polypeptide shows no membrane-spanning regions.