Related Experiment Videos
Location of divalent ion sites in acyl carrier protein using relaxation perturbed 2D NMR
A F Frederick1, L E Kay, J H Prestegard
1Department of Chemistry, Yale University, New Haven, CT 06511.
FEBS Letters
|September 26, 1988
Abstract:
The T1-accordion COSY experiment has been applied to acyl carrier protein (ACP) to locate the divalent ion binding sites in the protein using the paramagnetic ion, Mn2+, as a substitute for Ca2+. Replacement with Mn2+ leads to an enhancement of proton spin-lattice (T1) relaxation rates. These enhancements have a 1/r6 distance dependence that makes them extremely useful in structural analyses. Ion-proton distances ranging from 3.0 to 9.0 A have been obtained from this experiment and subsequently used as constraints in the molecular mechanics module of AMBER to refine a protein structure.