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Updated: Feb 1, 2026

A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity
Published on: August 9, 2017
Prenylated flavans from Daphne giraldii and their cytotoxic activities
Qian Sun1, Xin-Yue Shang2, Yu-Xi Wang2
1School of Traditional Chinese Materia Medica, Key Laboratory of Computational Chemistry-Based Natural Antitumor drug Research & Development, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, People's Republic of China; Xiamen Institute for Food and Drug Quality Control, Xiamen 361012, People's Republic of China.
Abstract:
Nine new prenylated flavan compounds with multi-chiral centers including two pairs of epimers were isolated from the stem and root bark of Daphne giraldii. Their structures were established by extensive NMR and HR-ESIMS spectroscopic data analyses. The in vitro cytotoxicity experiments indicated that compound 6 showed the most significant cytotoxicity against Hep3B cells, with an IC50 value of 9.83 μM. Hoechst 33258 and Annexin V-FITC/PI staining suggested that 6 could induce apoptosis of Hep3B cells in a concentration-dependent manner. Further mechanism study indicated that the apoptosis was associated with the up-regulations of Bax, cl-PARP and a decrease in Bcl-2 expression.
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