Related Experiment Video

Updated: Feb 1, 2026

Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus MRSA
12:18

Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus MRSA

Published on: February 9, 2011

23.6K

Genetic Diversity Analysis of Mupirocin-Resistant Staphylococcus aureus Clinical Isolates in Tehran Hospitals, Iran

Mehdi Goudarzi1, Maryam Fazeli2, Gita Eslami1

  • 11 Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Microbial Drug Resistance (Larchmont, N.Y.)
|December 1, 2018
PubMed

Insights

This study investigated mupirocin resistance in Staphylococcus aureus strains in Iran. Molecular characterization revealed the prevalence of resistance genes and identified specific clones, highlighting the need for monitoring.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Epidemiology

Background:

  • Mupirocin is a crucial antibiotic for treating Staphylococcus aureus infections.
  • Limited data exists on the molecular mechanisms of mupirocin resistance in S. aureus.
  • Understanding resistance patterns is vital for effective treatment strategies.

Purpose of the Study:

  • To characterize the molecular basis of mupirocin resistance in S. aureus isolates from Tehran, Iran.
  • To determine the prevalence of high-level and low-level mupirocin resistance.
  • To identify the genetic determinants and clonal types associated with mupirocin resistance.

Main Methods:

  • Cross-sectional study of 648 S. aureus isolates over one year.
  • Minimum inhibitory concentration (MIC) determination for mupirocin resistance levels.
  • Polymerase chain reaction (PCR) for detecting resistance genes (e.g., mupA).
  • Genotyping using S. aureus protein A (spa) and staphylococcal cassette chromosome mec (SCCmec) typing.
  • Multilocus sequence typing (MLST) for clonal analysis.

Main Results:

  • 51 mupirocin-resistant S. aureus strains were identified.
  • 23 isolates (45.1%) showed high-level mupirocin resistance (HLMUPR), and 28 (54.9%) showed low-level mupirocin resistance (LLMUPR).
  • The mupA gene was detected in 25 isolates (49%).
  • Seven distinct clones were identified, with specific ST-SCCmec-spa combinations associated with HLMUPR and LLMUPR phenotypes.
  • Two strains with the mupA gene exhibited LLMUPR, and one HLMUPR strain was Vancomycin-intermediate S. aureus (VISA).

Conclusions:

  • The study identified key molecular characteristics and clonal lineages of mupirocin-resistant S. aureus in Iran.
  • The presence of the mupA gene and specific clones are associated with mupirocin resistance.
  • Epidemiological monitoring and routine mupirocin susceptibility testing are essential for controlling resistance in methicillin-resistant S. aureus (MRSA).
Keywords:
typingSCC typingmethicillin-resistantmultilocus sequence typingmupirocinpolymerase chain reaction

More Related Videos

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR08:51

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR

Published on: November 4, 2016

10.2K
Development and Assessment of Intracellular Infection Models for Staphylococcus aureus08:32

Development and Assessment of Intracellular Infection Models for Staphylococcus aureus

Published on: January 17, 2025

1.3K

Related Concept Videos

Hospitals-II00:59

Hospitals-II

Hospitals provide inpatient and outpatient services. Inpatient services provide care to patients that stay in the hospital for an extended period, ranging from days to months. Examples of inpatient services include intensive care units, hospital wards, or surgeries. Outpatient services provide care to patients who come to a hospital for a diagnostic or treatment but do not stay overnight —for example, diagnostic tests, surgical procedures, or health education.
Nurses that work in...
1.2K
Hospitals-I01:28

Hospitals-I

Hospitals offer medical and surgical care to the sick and injured, along with accommodation while they recover. At the same time, they also provide outpatient, emergency, psychiatric, and rehabilitation services to meet various community needs. In addition to providing medical care, hospitals also act as hubs for medical research and training. Hospitals use clinical procedures and evidence-based practice standards to deliver patient care. To deliver safe and efficient care, a nurse must stay up...
1.6K
Genetics of Speciation02:16

Genetics of Speciation

Speciation is the evolutionary process resulting in the formation of new, distinct species—groups of reproductively isolated populations.
21.2K
Diversity of Archaea I01:30

Diversity of Archaea I

Archaea, a domain of single-celled microorganisms, are classified into five major phyla based on genetic and biochemical characteristics: Euryarchaeota, Crenarchaeota, Thaumarchaeota, Korarchaeota, and Nanoarchaeota. Among these, the phylum Euryarchaeota is notable for its remarkable diversity in morphology, metabolism, and ecological adaptations.Morphological and Metabolic DiversityMembers of Euryarchaeota exhibit a variety of cellular shapes, including rods and cocci. Their metabolic pathways...
628
Cell Diversity01:13

Cell Diversity

The concept of a cell started with microscopic observations of dead cork tissue by Robert Hooke in 1665. Hooke coined the term "cell" based on the resemblance of the small subdivisions in the cork to the rooms that monks inhabited, called cells. About ten years later, Antonie van Leeuwenhoek became the first person to observe the living and moving cells under a microscope. In the century that followed, the theory that cells represented the basic unit of life developed.
Multicellular...
5.1K
Diversity of Archaea II01:24

Diversity of Archaea II

Archaea, one of the three domains of life, exhibit remarkable diversity and adaptability, thriving in both extreme and moderate environments. Historically, most identified archaea have been classified into two major phyla: Euryarchaeota and Crenarchaeota. However, recent molecular studies have expanded this classification to include three additional phyla: Thaumarchaeota, Nanoarchaeota, and Korarchaeota, each exhibiting unique characteristics and ecological roles.Thaumarchaeota: Mesophiles...
524