[Relationship between Quantitative Monitoring of WT1 Gene and Prognosis in Patients with Acute Leukemia]
Related Experiment Video
Updated: Feb 1, 2026

In Ovo Xenografting of Patient-Derived Acute Lymphoblastic Leukemia (ALL) Cells (PDX-ALL)
Published on: August 1, 2025
Cui-Yun Dou1, Ji-Lei Zhang2, Qian Huang2
1Department of Hematology, The Affiliated Hospital of Jining Medical College, Jining 272029, Shandong Province, China.E-mail: 15866061657@163.com.
Objective:
To investigate the exprassion of WT1 gene in patients with adult acute myeloid leukemia (AML) and its clinical significance.
Methods:
Sixty-three newly diagnosed patients with acute myeloid leukemia were selected. Quantitative RT-PCR was used to detect the expression of WT1 gene in the 63 AML patients and 20 non-AML controls.
Results:
WT1 gene was highly expressed in AML patents and its expression in the low-risk group was significantly lower than that in middle-risk group and high-risk group (P<0.05), and no significant difference of WT1 gene expression between middle-risk and high-risk group was observed. In the patients of middle-risk and high-risk patients, the expression of WT1 gene in the remission group was significantly lower than that in the patients of non-remission after treatment (P<0.05). The non-remission patients after first treatment in middle-risk and high-risk group were treated with second induction therapy. After second induction therapy, the WT1 expression in remission patients was significantly decreased (P<0.05) in comparison with that in patients still in non-remission. There was a negative correlation between WT1 expression and the 2-year overall survival rate in the newly diagnosed middle and high-risk AML patients.
Conclusion:
The detection of WT1 gene expression can help to divide AML patients into low-/middle-/high-risk groups and to evaluate therapeutic response and clinical prognosis in middle and high-risk AML patients.
More Related Videos
08:31Murine Model of Leukemia Relapse to Induction Chemotherapy for Acute Lymphoblastic Leukemia
Published on: October 17, 2025
09:01Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
Published on: March 26, 2018
Related Concept Videos
Relationship Formation
Ending Relationships
Relationship Growth
Social Relationships and Well-Being
Close Relationships and Culture
Gene Evolution - Fast or Slow?
In contrast, regions which code...