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Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia
09:23

Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia

Published on: August 14, 2017

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Differential suppression of persistent insect specific viruses in trans-infected wMel and wMelPop-CLA Aedes-derived

Breeanna J McLean1, Kimberley R Dainty1, Heather A Flores1

  • 1Institute of Vector-Borne Disease, Monash University, Clayton, Vic., Australia.

Virology
|December 4, 2018
PubMed

Insights

Wolbachia bacteria can suppress insect-specific viruses in mosquito cell cultures, particularly positive-strand RNA viruses like Cell-fusing agent virus, but not negative-strand RNA viruses.

Area of Science:

  • Microbiology
  • Virology
  • Entomology

Background:

  • Wolbachia endosymbionts are known to suppress replication of certain viruses, including dengue and Zika, in Aedes aegypti mosquitoes.
  • The full spectrum of viruses impacted by Wolbachia, especially novel insect-specific viruses (ISVs), remains largely unexplored.

Purpose of the Study:

  • To investigate the effect of Wolbachia on the replication of insect-specific viruses in mosquito cell lines.
  • To determine if Wolbachia can suppress Cell-fusing agent virus (CFAV) and Phasi Charoen-like virus (PCLV) infections.

Main Methods:

  • Utilized Aedes aegypti (Aag2) and Aedes albopictus (RML-12) cell lines infected with different strains of Wolbachia (wMel, wMelPop-CLA).
  • Assessed viral replication of CFAV (a Flaviviridae) and PCLV (a Bunyaviridae) in these cell lines.
  • Detected viral presence and replication in cell cultures under Wolbachia infection.

Main Results:

  • Wolbachia restricted the replication of Cell-fusing agent virus (CFAV) in Aag2 cells.
  • Phasi Charoen-like virus (PCLV) replication was not suppressed by Wolbachia in Aag2 cells.
  • PCLV infection was identified in RML-12 cells containing Wolbachia, with no clear suppression observed.

Conclusions:

  • Wolbachia can suppress insect-specific, positive-sense single-stranded RNA viruses (+ssRNA) in mosquito cell culture.
  • Negative-sense single-stranded RNA viruses (-ssRNA) may not be suppressed by Wolbachia.
  • These findings expand the understanding of Wolbachia's antiviral capabilities against diverse insect viruses.
Keywords:
BunyavirusFlavivirusInsect specific virusesMosquito cell linesWolbachia

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