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A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
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High-throughput screening for small molecule inhibitors of the type-I interferon signaling pathway

Elita Yuliantie1,2, Xinchuan Dai1, Dehua Yang1,2

  • 1The National Center for Drug Screening and the CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences (CAS), Shanghai 201203, China.

Acta Pharmaceutica Sinica. B
|December 4, 2018
PubMed

Keywords:
CV, coefficient of variationDMEM, Dulbecco׳s modified Eagle׳s mediumDMSO, dimethyl sulfoxideFRET, fluorescence resonance energy transferHEK, human embryonic kidneyHTS, high-throughput screeningHigh-throughput screeningIFN regulatory factorIFN, interferonIFNAR, IFN alpha receptorIRF, IFN regulatory factorISGF3, IFN-stimulated gene factor 3ISRE, IFN-stimulated response elementInhibitorInterferon α receptorJAK, Janus activated kinaseJAK-STATS/B, signal to background ratioSEAP, secreted embryonic alkaline phosphataseSTAT, signal transducer and activator of transcriptionSecreted embryonic alkaline phosphataseTYK, tyrosine kinasecDNA, complementary DNApSTAT, phosphorylated STAT

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