Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes02:16

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

16.1K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
16.1K
DNA Microarrays02:34

DNA Microarrays

21.1K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
21.1K
Chromosome Replication02:31

Chromosome Replication

10.6K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
10.6K
Polytene Chromosomes02:04

Polytene Chromosomes

11.0K
Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also...
11.0K
Chromosome Structure02:40

Chromosome Structure

26.5K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
26.5K
Lampbrush Chromosomes01:51

Lampbrush Chromosomes

8.7K
In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...
8.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Management of Recurrent Venous Thromboembolism on Anticoagulation.

Journal of clinical medicine·2026
Same author

Malaria-associated splenic haemorrhage requiring emergency splenectomy: a rare complication managed in a resource-constrained setting.

Malaria journal·2026
Same author

Nursing Perspectives on Factors That Influence Provision of Patient-Centered Care for Autistic Patients in a Large Urban Hospital System: A Qualitative Study.

Journal of advanced nursing·2026
Same author

Maternal and Fetal Outcomes in Gestational Diabetes Mellitus Treated with Metformin with or Without Insulin.

Journal of obstetrics and gynaecology of India·2025
Same author

Single high-dose amphotericin B for cryptococcal meningitis: First Indian experience.

Medical journal, Armed Forces India·2025
Same author

The Extent to Which Artificial Intelligence Can Help Fulfill Metastatic Breast Cancer Patient Healthcare Needs: A Mixed-Methods Study.

Current oncology (Toronto, Ont.)·2025
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Feb 1, 2026

Array Comparative Genomic Hybridization Array CGH for Detection of Genomic Copy Number Variants
09:16

Array Comparative Genomic Hybridization Array CGH for Detection of Genomic Copy Number Variants

Published on: February 21, 2015

20.5K

Chromosomal Microarray Analysis Using Array Comparative Genomic Hybridization on DNA from Amniotic Fluid and

Ankita Patel1

  • 1Lineagen, Salt Lake City, UT, USA. ankita.patel@lineagen.com.

Methods in Molecular Biology (Clifton, N.J.)
|December 4, 2018
PubMed
Summary
This summary is machine-generated.

Chromosomal Microarray analysis provides a high-resolution view of genomic copy number changes. This study presents a rapid method for detecting genomic imbalances linked to congenital malformations in prenatal samples.

Keywords:
Array CGHComparative genomic hybridizationMicroarrayPrenatal diagnosis

More Related Videos

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

38.6K
Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
16:37

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

13.3K

Related Experiment Videos

Last Updated: Feb 1, 2026

Array Comparative Genomic Hybridization Array CGH for Detection of Genomic Copy Number Variants
09:16

Array Comparative Genomic Hybridization Array CGH for Detection of Genomic Copy Number Variants

Published on: February 21, 2015

20.5K
DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

38.6K
Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
16:37

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

13.3K

Area of Science:

  • Genetics
  • Genomics
  • Molecular Biology

Background:

  • Chromosomal Microarray (CMA) analysis is a powerful tool for detecting genomic copy number variations (CNVs).
  • CNVs are implicated in various genetic disorders across postnatal, prenatal, and oncology settings.
  • Accurate detection of genomic imbalances is crucial for diagnosing genetic conditions.

Purpose of the Study:

  • To describe a rapid and reliable method for chromosomal microarray analysis.
  • To apply this method for detecting genomic imbalances associated with congenital malformations in prenatal diagnosis.
  • To establish a timely diagnostic approach for fetal genetic abnormalities.

Main Methods:

  • Utilized chromosomal microarray analysis (CMA) for high-resolution genomic profiling.
  • Employed direct analysis of amniotic fluid (AF) and chorionic villus samples (CVS).
  • Optimized protocols for rapid turnaround time.

Main Results:

  • Demonstrated the effectiveness of CMA in identifying genomic imbalances in prenatal samples.
  • Achieved results within a 4-5 day timeframe.
  • Validated the reliability of the method for clinical application.

Conclusions:

  • The described CMA method offers a fast and reliable approach for prenatal diagnosis.
  • This technique aids in the detection of genomic imbalances linked to congenital malformations.
  • Accelerated CMA analysis can significantly improve prenatal genetic testing.