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Noninvasive Antenatal Screening for Fetal RHD in RhD Negative Women to Guide Targeted Anti-D Prophylaxis

Frederik Banch Clausen1, Klaus Rieneck2, Grethe Risum Krog2

  • 1Department of Clinical Immunology, Section 2034, Copenhagen University Hospital, Copenhagen, Denmark. Frederik.banch.clausen@regionh.dk.

Methods in Molecular Biology (Clifton, N.J.)
|December 4, 2018
PubMed

RhD negative pregnant women who carry an RhD positive fetus are at risk of immunization against the D antigen, which may result in hemolytic disease of the fetus and the newborn. Predicting the fetal RhD status by noninvasive antenatal screening for the fetal RhD gene (RHD) can guide targeted use of antenatal anti-D prophylaxis.Cell-free fetal DNA is extracted from maternal plasma from RhD negative pregnant women at a gestational age of 25 weeks. A real-time PCR-based detection of two RHD exons enables reliable prediction of the fetal RhD status to determine the administration of antenatal prophylaxis, as well as postnatal prophylaxis.

Keywords:
Antenatal prophylaxisAnti-DCell-free fetal DNAHemolytic disease of the fetus and newbornNoninvasive RHD typingReal-time PCR

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