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Published on: June 25, 2010
Assaying D-Body Amino Acids as D-Alanine and Amino Acid Racemase (AARase) Activity Using NADH Oxidoreduction Enzymic
Natsuki Matsumoto1, Makoto Kanauchi2
1Department of Food Management, Miyagi University, Sendai, Miyagi, Japan.
Abstract:
Fermented foods produced using edible microorganisms have been consumed worldwide since ancient times. Microorganisms produce some healthy functional substances. Recently, many people have been attracted to the notion that D-body amino acids have healthy functional effects. D-body amino acids convert L-body amino acid proteolysis from a substrate such as foods during fermentation. This chapter presents a description of methods used for D-alanine assays in the solution as medium and AARase assays in lactic acid bacteria (LAB) cells for isolation of high AARase-containing strains using D-amino acid oxidase and lactic acid dehydrogenase via a NADH oxidoreduction system.
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