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Updated: Jun 14, 2025
Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
Separation of Lactic Acid Bacteria Using Blue-Native (BN) Polyacrylamide Gel Electrophoresis (PAGE) and Analyses of
1Department of Food Management, Miyagi University, Sendai, Japan. kanauchi@myu.ac.jp.
Abstract:
Some gram-negative bacteria such as Escherichia coli and Salmonella spp. among intestinal bacteria might induce inflammation of human intestines. However, lactic acid bacteria (LAB) display anti-inflammatory activity, which improves the intestinal environment. Particularly, the cell surface protein on Pediococcus pentosaceus exhibits high LPS elimination activity, which is expected to provide anti-inflammatory activity in the intestines. This chapter describes that surface proteins are separable using Blue-Native PAGE, which relies upon the theory that protein binds with Coomassie brilliant blue to produce a negative charge for easy separation.
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