Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Affinity and Avidity01:41

Affinity and Avidity

39.0K
Overview
39.0K
Electron Affinity03:07

Electron Affinity

43.3K
The electron affinity (EA) is the energy change for adding an electron to a gaseous atom to form an anion (negative ion).
43.3K
The Tumor Microenvironment02:17

The Tumor Microenvironment

7.8K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
7.8K
Laminins are the Adhesive Proteins of Basal Lamina00:55

Laminins are the Adhesive Proteins of Basal Lamina

3.2K
Laminins are heterotrimeric proteins with high molecular mass found in the extracellular matrix. Each laminin molecule is composed of three chains, viz. alpha, beta, and gamma, coded by five, four, and three paralogous genes, respectively. Laminins are categories based on the compositions of the three chains.
In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...
3.2K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.2K
Overview
80.2K
Affinity Chromatography01:03

Affinity Chromatography

3.0K
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-nuclei UPR profiling by flow cytometry reveals bortezomib resistance mechanisms in multiple myeloma.

EMBO molecular medicine·2026
Same author

Prediction of Mesenchymal Stromal Cell Immune Suppression Using Live Imaging in a Three-Dimensional Microfluidic Device.

ACS biomaterials science & engineering·2026
Same author

A microgel bone marrow model of mesenchymal stromal cell paracrine signaling supporting hematopoietic stem cell retention.

Acta biomaterialia·2026
Same author

Bone marrow-derived mesenchymal stromal cells yield greater pain relief and tissue protection than umbilical cord tissue-derived cells in a surgically induced instability model of osteoarthritis.

Osteoarthritis and cartilage·2026
Same author

Professor Xingdong Zhang Special Issue.

Tissue engineering. Part A·2026
Same author

Guiding Principles: Reporting Elements for Gastrointestinal Organoid Research.

Cellular and molecular gastroenterology and hepatology·2026

Related Experiment Video

Updated: Feb 1, 2026

Generating a Fractal Microstructure of Laminin-111 to Signal to Cells
06:56

Generating a Fractal Microstructure of Laminin-111 to Signal to Cells

Published on: September 28, 2020

1.3K

An affinity-based approach to engineer laminin-presenting cell instructive microenvironments.

Daniela Barros1, Paula Parreira2, Joana Furtado3

  • 1i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto (UPorto), Portugal; INEB - Instituto de Engenharia Biomédica, UPorto, Portugal; ICBAS - Instituto de Ciências Biomédicas Abel Salazar, UPrto, Portugal.

Biomaterials
|December 5, 2018
PubMed
Summary
This summary is machine-generated.

A new affinity-based method allows site-specific immobilization of laminin, preserving its bioactivity for improved stem cell applications. This approach enhances neural stem cell adhesion and spreading on engineered surfaces.

Keywords:
Affinity-bindingAgrinCell microenvironmentLamininProtein immobilizationSelf-assembled monolayers

More Related Videos

Experimental Approaches to Tissue Engineering
16:41

Experimental Approaches to Tissue Engineering

Published on: August 30, 2007

6.8K
Engineering Cell-permeable Protein
21:08

Engineering Cell-permeable Protein

Published on: December 28, 2009

15.0K

Related Experiment Videos

Last Updated: Feb 1, 2026

Generating a Fractal Microstructure of Laminin-111 to Signal to Cells
06:56

Generating a Fractal Microstructure of Laminin-111 to Signal to Cells

Published on: September 28, 2020

1.3K
Experimental Approaches to Tissue Engineering
16:41

Experimental Approaches to Tissue Engineering

Published on: August 30, 2007

6.8K
Engineering Cell-permeable Protein
21:08

Engineering Cell-permeable Protein

Published on: December 28, 2009

15.0K

Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Extracellular Matrix Engineering

Background:

  • Laminin immobilization is crucial for replicating stem cell niche microenvironments.
  • Controlling laminin orientation and bioactivity during immobilization remains a significant challenge.
  • Existing methods often fail to preserve key bioactive epitopes of laminin.

Purpose of the Study:

  • To develop an affinity-based strategy for site-specific laminin immobilization.
  • To preserve laminin's bioactivity and orientation on engineered substrates.
  • To evaluate the effectiveness of this novel immobilization technique for stem cell applications.

Main Methods:

  • Utilized the high-affinity interaction between laminin and the human N-terminal agrin (hNtA) domain.
  • Produced recombinant hNtA (rhNtA) and conjugated it to thiol-terminated poly(ethylene glycol) (PEG).
  • Prepared self-assembled monolayers (SAMs) of mono-PEGylated rhNtA on gold (mPEG rhNtA-SAMs) for laminin immobilization.

Main Results:

  • Site-specific immobilization of laminin onto mPEG rhNtA-SAMs preserved protein bioactivity.
  • This method showed enhanced self-polymerization and mediation of human neural stem cell adhesion and spreading compared to non-selective immobilization.
  • Laminin bioactivity was better maintained using the affinity-based approach.

Conclusions:

  • The novel affinity-based approach offers a superior alternative to conventional laminin immobilization methods.
  • This strategy effectively preserves laminin's bioactivity and orientation.
  • Potential applications include engineered coatings for neuroelectrodes, cell culture, and 3D matrix biofunctionalization.