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Related Experiment Video

Updated: Feb 1, 2026

Heterogeneous Removal of Water-Soluble Ruthenium Olefin Metathesis Catalyst from Aqueous Media Via Host-Guest Interaction

Heterogeneous Removal of Water-Soluble Ruthenium Olefin Metathesis Catalyst from Aqueous Media Via Host-Guest Interaction

Published on: August 23, 2018

Quantifying Guest-Host Dynamics in Supramolecular Assemblies to Analyze Their Robustness.

Maartje M C Bastings^1,2,3, Thomas M Hermans^1,2,4, A J H Spiering^1,5

¹Institute for Complex Molecular Systems, Eindhoven University of Technology, PO Box 513,, 5600, MB Eindhoven, The Netherlands.

Macromolecular Bioscience

|December 5, 2018

Summary

Synthetic microenvironments in tissue engineering require dynamic cell interactions. Researchers balanced material functionalization and cell dynamics using ureido-pyrimidinone (UPy) systems, finding divalent guests offer stronger interactions but risk leakage.

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Synthesis and Characterization of Supramolecular Colloids

Synthesis and Characterization of Supramolecular Colloids

Published on: April 22, 2016

Area of Science:

Biomaterials Science
Tissue Engineering
Supramolecular Chemistry

Background:

Synthetic microenvironments are crucial for cell attachment, migration, and proliferation in tissue engineering.
Functionalizing supramolecular materials with guest compounds introduces bioactivity.
A balance between robust material interaction and dynamic cell engagement is essential for adaptive interfaces.

Purpose of the Study:

To analyze the dynamic interactions within a ureido-pyrimidinone (UPy) functionalized poly(ethylene glycol) system.
To evaluate the impact of monovalent and bivalent guest molecule design on material-cell interactions.
To establish a method for quantifying guest mobility in dilute and gel states for adaptive biomaterial design.

Main Methods:

Utilized a ureido-pyrimidinone (UPy) poly(ethylene glycol) system in dilute and transient network regimes.
Designed and evaluated monovalent and bivalent UPy-functionalized guest molecules interacting with UPy-host fibers.
Employed microfluidics for dilute state analysis and fluorescence recovery after photobleaching (FRAP) and fluorescence resonance energy transfer (FRET) for gel state mobility quantification.

Main Results:

Supramolecular host-guest chemistry enables dynamic interactions of bioactive moieties.
Divalent guests exhibit stronger interactions compared to monovalent guests.
While stronger interactions are beneficial, there is a risk of unintended leakage with functional monomeric units.

Conclusions:

The study demonstrates a method to achieve dynamic functionalization in synthetic microenvironments.
Balancing guest valency is key to optimizing interaction strength while minimizing leakage.
This approach provides insights for designing adaptive and bioactive interfaces for tissue engineering applications.