Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Helicases00:55

DNA Helicases

24.1K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
24.1K
Manipulation and Analysis01:21

Manipulation and Analysis

301
GIS manipulation and analysis functions are vital for decision-making and planning. These activities range from data retrieval tasks, such as selecting information based on specific criteria, to advanced analytical techniques that address complex spatial problems.One critical GIS analysis method is overlaying, which combines multiple data layers to examine impacts. For example, overlaying a river-dammed lake boundary with road networks can identify affected infrastructure. Another common...
301
DNA Replication02:40

DNA Replication

59.3K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
59.3K
Viral Structure00:56

Viral Structure

74.5K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
74.5K
From DNA to Protein03:06

From DNA to Protein

22.4K
The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
22.4K
The DNA Helix01:16

The DNA Helix

157.5K
Overview
157.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A pH-Responsive Topological Switch Based on a DNA Quadruplex-Duplex Hybrid.

Chemistry (Weinheim an der Bergstrasse, Germany)·2024
Same author

Structural Differences at Quadruplex-Duplex Interfaces Enable Ligand-Induced Topological Transitions.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2024
Same author

Impact of loop length and duplex extensions on the design of hybrid-type G-quadruplexes.

Chemical communications (Cambridge, England)·2023
Same author

Showcasing Different G-Quadruplex Folds of a G-Rich Sequence: Between Rule-Based Prediction and Butterfly Effect.

Journal of the American Chemical Society·2023
Same author

Simulations of electric field gradient fluctuations and dynamics around sodium ions in ionic liquids.

The Journal of chemical physics·2022
Same author

Influence of alkali metals on water dynamics inside imidazolium-based ionic liquid nano-domains.

Frontiers in chemistry·2022

Related Experiment Video

Updated: Feb 1, 2026

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.8K

Manipulating DNA G-Quadruplex Structures by Using Guanosine Analogues.

Linn Haase1, Beatrice Karg1, Klaus Weisz1

  • 1Institute of Biochemistry, Universität Greifswald, Felix-Hausdorff-Strasse 4, 17487, Greifswald, Germany.

Chembiochem : a European Journal of Chemical Biology
|December 5, 2018
PubMed
Summary

Controlling DNA G-quadruplex folding topology is key for designing novel scaffolds. Specific modifications to guanosine surrogates can induce conformational changes, enabling new therapeutic and technological applications.

Keywords:
G-quadruplexesaptamersconformation analysisguanosineoligonucleotidestopology

More Related Videos

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.6K
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.3K

Related Experiment Videos

Last Updated: Feb 1, 2026

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.8K
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.6K
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.3K

Area of Science:

  • Biochemistry and Molecular Biology
  • Structural Biology

Background:

  • DNA G-quadruplexes are unique nucleic acid structures with potential therapeutic and technological applications.
  • Controlling their folding topology is crucial for rational design.
  • Understanding the impact of chemical modifications on G-quadruplex structure is an ongoing challenge.

Purpose of the Study:

  • To investigate how specific base- and sugar-modified guanosine surrogates influence DNA G-quadruplex folding topology.
  • To explore the induction of conformational transitions and refolding into new topologies through judicious incorporation of modified nucleosides.

Main Methods:

  • Systematic substitution experiments with modified guanosine surrogates.
  • High-resolution structural analysis of modified G-quadruplex variants.

Main Results:

  • Judicious incorporation of modified guanosine surrogates can induce conformational transitions in G-quadruplexes.
  • These transitions include tetrad polarity inversions and complete refolding into new topologies.
  • Emerging evidence highlights the importance of specific interactions and glycosidic bond angle propensities in modification-driven refolding.

Conclusions:

  • Chemical modifications offer a powerful strategy to control DNA G-quadruplex folding topology.
  • This control enables the rational design of G-quadruplex-based scaffolds for diverse applications.
  • Further research into the intricate interactions governing G-quadruplex stability and refolding is essential.