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[Effect of germ cell Wdr1 deletion on the ovarian function of mice]

Zhiyun Shi1, Juan Xu, Xuemei Jia

  • 1Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Jiangsu, Nanjing 210004, China. xmjia@njmu.edu.cn.

Zhonghua Yi Xue Yi Chuan Xue Za Zhi = Zhonghua Yixue Yichuanxue Zazhi = Chinese Journal of Medical Genetics
|December 5, 2018
PubMed
Abstract

Insights

Germ cell-specific deletion of the Wdr1 gene in mice leads to reduced ovarian volume and follicular numbers, ultimately causing premature ovarian failure and impacting early ovarian function.

Area of Science:

  • Reproductive Biology
  • Genetics
  • Developmental Biology

Background:

  • The Wdr1 gene plays a crucial role in actin dynamics, essential for various cellular processes.
  • Understanding the role of Wdr1 in germ cells is vital for comprehending ovarian development and function.

Purpose of the Study:

  • To investigate the impact of Wdr1 gene deletion specifically in germ cells on mouse ovarian function.
  • To determine if Wdr1 deficiency in oocytes contributes to ovarian dysfunction or failure.

Main Methods:

  • An oocyte-specific Wdr1 knockout mouse model (Wdr1fl/-; Ddx4-Cre) was generated.
  • Ovaries were analyzed at 14 days, 28 days, and 4 months post-birth using gross photography, paraffin sectioning, and Hematoxylin-Eosin (HE) staining.
  • Ovarian volume and follicle counts were quantified to assess ovarian health and development.

Main Results:

  • A slight reduction in ovarian volume and follicle counts (primordial, primary, secondary) was observed at 14 days in Wdr1-deficient mice compared to controls.
  • Significant decreases in ovarian volume and the number of all developmental follicles were evident at 28 days and 4 months.
  • These findings indicate a progressive decline in ovarian function due to Wdr1 deletion.

Conclusions:

  • Germ cell-specific deletion of Wdr1 significantly impairs ovarian function in mice.
  • Wdr1 deficiency in germ cells is implicated in the development of premature ovarian failure.
  • The study highlights the essential role of Wdr1 in maintaining ovarian health and reproductive capacity.

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