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Solution-Controlled Conformational Switching of an Anchored Wireframe DNA Nanostructure
Ian T Hoffecker1, Sijie Chen1,2, Andreas Gådin1
1Biomaterials, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Solnvägen 9, 171 65, Solna, Sweden.
Small (Weinheim an Der Bergstrasse, Germany)
|December 6, 2018
Summary
Researchers developed a flexible DNA nanomesh that dynamically transitions between spread and contracted states on surfaces. This DNA origami nanostructure offers new possibilities for controlling and observing nanoscale interactions.
Area of Science:
- Nanotechnology
- Biophysics
- Materials Science
Background:
- DNA origami nanostructures offer precise spatial control for nanoscale manipulations.
- Flexible DNA nanostructures can exhibit dynamic conformational changes.
Purpose of the Study:
- To report a surface-tethered, flexible DNA nanomesh that undergoes spontaneous conformational transitions.
- To investigate the dynamics and control of these transitions under physiological conditions.
Main Methods:
- Utilized DNA PAINT microscopy for in situ observation of dynamic conformations.
- Employed time-series localization data and automated image processing.
- Applied dynamic light scattering to study solution interactions.
Main Results:
- The DNA nanomesh transitions between a surface-interacting spread state and a surface-avoiding contracted state.
- Transitions occur stochastically over tens of minutes and are detectable in situ.
- Calcium ions and elevated pH influence the conformational distribution, favoring the spread state via electrostatic interactions with surface proteins (BSA/streptavidin).
Conclusions:
- The developed DNA nanomesh exhibits controllable, dynamic conformational transitions.
- This system provides a platform for observing and controlling nanoscale interactions and spatial relationships.
- The findings suggest potential applications in nanoscale molecular manipulation and sensing.
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