Related Experiment Videos
[Instability of immunoglobulin expression during differentiation induction in the human lymphoblastoid cell line
The induction of immunoglobulin heavy chain classes switch from IgM to IgG was demonstrated in vitro in cells of RPMI-6410t line. The IgG+-sublines, formed as a result of the switch are characterized by instability of IgG synthesis. After removal of the inductor from the growth environment, IgG+ cells gradually reduce the level of secreted IgG. Such a transition to the functional rest state is likely to be connected with the convertible Ig-gene activity suppression in IgG+ cells, since after their activation by LPS IgG-secretion is partly or completely restored. The IgG+-sublines obtained may serve a convenient model for investigating the Ig-gene expression regulation in differentiated human B-cells.
The induction of immunoglobulin heavy chain classes switch from IgM to IgG was demonstrated in vitro in cells of RPMI-6410t line. The IgG+-sublines, formed as a result of the switch are characterized by instability of IgG synthesis. After removal of the inductor from the growth environment, IgG+ cells gradually reduce the level of secreted IgG. Such a transition to the functional rest state is likely to be connected with the convertible Ig-gene activity suppression in IgG+ cells, since after their activation by LPS IgG-secretion is partly or completely restored. The IgG+-sublines obtained may serve a convenient model for investigating the Ig-gene expression regulation in differentiated human B-cells.