Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

16.8K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.8K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.9K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.9K
Complex Numbers01:29

Complex Numbers

309
The real number system cannot represent the square root of a negative number, which restricts solutions for certain equations, such as quadratics with negative discriminants. To address this, the complex number system was developed, introducing the imaginary unit i, where i = √(-1). This extension allows for the representation of all roots, including those involving negative radicands.A complex number is written in the form x + yi, where x and y are real numbers. Here, x represents the...
309
Formation of Complex Ions03:45

Formation of Complex Ions

26.1K
A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
26.1K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

8.6K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.6K
Complex Power01:14

Complex Power

916
Power engineers have introduced the concept of complex power to determine the cumulative effect of parallel loads. This idea plays a crucial role in power analysis because it encompasses all the details related to the power consumed by a specific load.
Complex power is defined as the multiplication of the voltage and the complex conjugate of the current. The magnitude of this power, known as apparent power, is measured in volt-amperes (VA). Notably, the angle of the complex power equates to the...
916

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metal-Organic Frameworks as Programable Platforms for Micro- and Optoelectronics: From Dielectrics to Semiconductors and White-Light Emitters.

Small science·2026
Same author

Structural basis for asymmetric bis-intercalator targeting of DNA triplex junctions enabling dual inhibition of topoisomerase I and oncogene transcription.

Nucleic acids research·2026
Same author

PROTAC-Mediated Degradation of mHTT Aggregates Attenuates Neurotoxicity in Cellular and R6/2 Mouse Models of Huntington's Disease.

Journal of the American Chemical Society·2026
Same author

Single-cell and spatial omics reveal progressive loss of xylem developmental complexity across seed plants.

The Plant cell·2025
Same author

Synergistic Composite Solid Electrolyte Based on Multifunctional Polymer Networks for High-Performance Lithium Metal Batteries.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Single-cell and spatial multiomics identifies heterogeneous xylem development driven by mechanical stress in Populus.

Developmental cell·2025

Related Experiment Video

Updated: Feb 1, 2026

Detection of Signaling Effector-Complexes Downstream of BMP4 Using in situ PLA, a Proximity Ligation Assay
12:52

Detection of Signaling Effector-Complexes Downstream of BMP4 Using in situ PLA, a Proximity Ligation Assay

Published on: March 3, 2011

32.2K

Dioxygen activation by a dinuclear thiolate-ligated Fe(ii) complex.

Chang-Chih Hsieh1, Yu-Chiao Liu, Mei-Chun Tseng

  • 1Department of Chemistry, National Changhua University of Education, Changhua 50058, Taiwan. ychorng@cc.ncue.edu.tw.

Dalton Transactions (Cambridge, England : 2003)
|December 6, 2018
PubMed
Summary

Dioxygen activation by iron-sulfur complexes is challenging. This study shows a dinuclear iron complex with cis-thiolate activates O2 to form a mononuclear iron complex and water, avoiding sulfur oxidation.

More Related Videos

Knowledge Based Cloud FE Simulation of Sheet Metal Forming Processes
11:05

Knowledge Based Cloud FE Simulation of Sheet Metal Forming Processes

Published on: December 13, 2016

12.7K
In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
08:56

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

Published on: May 5, 2020

6.3K

Related Experiment Videos

Last Updated: Feb 1, 2026

Detection of Signaling Effector-Complexes Downstream of BMP4 Using in situ PLA, a Proximity Ligation Assay
12:52

Detection of Signaling Effector-Complexes Downstream of BMP4 Using in situ PLA, a Proximity Ligation Assay

Published on: March 3, 2011

32.2K
Knowledge Based Cloud FE Simulation of Sheet Metal Forming Processes
11:05

Knowledge Based Cloud FE Simulation of Sheet Metal Forming Processes

Published on: December 13, 2016

12.7K
In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
08:56

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

Published on: May 5, 2020

6.3K

Area of Science:

  • Bioinorganic Chemistry
  • Organometallic Chemistry
  • Catalysis

Background:

  • Dioxygen (O2) activation by iron(II) thiolate complexes is rare due to oxidative instability of the sulfur site.
  • O2 activation typically yields Fe(IV)=O intermediates or S-oxygenated products, depending on thiolate coordination (trans vs. cis).
  • The Fe(IV)=O intermediate, crucial in some enzymes, has only recently been observed spectroscopically.

Purpose of the Study:

  • To investigate O2 activation by a dinuclear Fe(II) thiolate-rich complex.
  • To explore the reaction pathway and intermediates formed, particularly when thiolate is cis to the O2 binding site.
  • To understand mechanisms that prevent oxidative damage to the thiolate ligand.

Main Methods:

  • Reaction of a dinuclear Fe(II) thiolate complex with O2 in the presence of electron donors.
  • Characterization of reaction products using mass spectrometry (cold-spray ionization time-of-flight mass spectrometry - CSI-TOF MS) and low-temperature UV-vis spectroscopy.
  • Theoretical calculations (Density Functional Theory - DFT) to probe reaction mechanisms and intermediate structures.

Main Results:

  • O2 activation produced a mononuclear Fe(III) complex and water, with no observed Fe(III)-O-Fe(III) or S-oxygenated species.
  • An unprecedented Fe(III)-O2-Fe(III) complex was detected by CSI-TOF MS and supported by UV-vis measurements.
  • DFT calculations suggested the transient existence of an Fe(IV)=O intermediate stabilized by intramolecular N-H···O-Fe hydrogen bonding, facilitating hydrogen abstraction and preventing S-oxygenation.

Conclusions:

  • A dinuclear Fe(II) thiolate complex can activate O2 to form a mononuclear Fe(III) complex and water, with cis-thiolate coordination.
  • The reaction pathway avoids S-oxygenation, likely due to intramolecular hydrogen bonding stabilizing a transient Fe(IV)=O intermediate.
  • This work provides insights into controlled dioxygen activation by iron-thiolate systems, relevant to biological and chemical catalysis.