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Genetic Variation in Intergenic and Exonic miRNA Sequence and Risk of Multiple Sclerosis in the Isfahan Patients

Zeynab Golshani1, Zohreh Hojati, Ali Sharifzadeh

  • 1Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran AND Department of Biology, Fars Science and Research Branch, Islamic Azad University, Shiraz, Iran. zey_golshani@yahoo.com.

Iranian Journal of Allergy, Asthma, and Immunology
|December 7, 2018
PubMed

Insights

Single nucleotide polymorphisms in microRNA-23a (mir23a) are associated with an increased risk of multiple sclerosis (MS) in Iranian patients. This finding suggests mir23a rs3745453 may contribute to MS etiology.

Area of Science:

  • Genetics
  • Immunology
  • Neurology

Background:

  • MicroRNAs (miRNAs) play a crucial role in the pathogenesis of multiple sclerosis (MS), a chronic inflammatory autoimmune disease.
  • Single nucleotide polymorphisms (SNPs) in miRNA sequences can alter their expression and function, potentially leading to disease.
  • Previous studies suggest a link between miRNA polymorphisms and increased risk of autoimmune diseases.

Purpose of the Study:

  • To investigate the association between specific miRNA polymorphisms (mir155 rs767649, mir196a2 rs11614913, and mir23a rs3745453) and the risk of multiple sclerosis in an Iranian population.
  • To determine if these miRNA polymorphisms contribute to the genetic susceptibility of MS in Isfahan.

Main Methods:

  • A case-control study involving 80 MS patients and 80 healthy controls from Isfahan, Iran.
  • DNA extraction followed by genotyping using the tetra amplification refractory mutation system-PCR (T ARMS PCR) method.
  • Analysis of genotype and allele frequencies for mir155, mir196a2, and mir23a polymorphisms.

Main Results:

  • The mir23a rs3745453 polymorphism showed a significant association with MS risk. Individuals with the TC genotype had a 2.3-fold increased risk (OR=2.3, p=0.048).
  • No significant differences in genotype or allele frequencies were observed for mir155 rs767649 and mir196a2 rs11614913 between MS patients and healthy controls (p>0.05).
  • CT heterozygosity in the mir23a gene was significantly related to the risk of developing MS.

Conclusions:

  • The mir23a rs3745453 polymorphism, specifically CT heterozygosity, is significantly associated with an increased risk of multiple sclerosis in the studied Iranian population.
  • Unlike mir155 and mir196a2, mir23a rs3745453 may play a role in the etiology of MS in Isfahan.
  • Further extensive research is warranted to validate these findings and establish a more definitive link between mir23a polymorphism and MS pathogenesis.

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