Author Correction: Efficient genetic engineering of human intestinal organoids using electroporation

Area of Science:

• Molecular Biology
• Biotechnology

Context:

• This correction addresses errors in a published scientific protocol.
• Accurate reagent concentrations are crucial for reproducible experimental outcomes.

Purpose:

• To rectify incorrect units for plasmid concentrations in a published protocol.
• To ensure clarity and accuracy in molecular biology experimental procedures.

Summary:

• The protocol for sgRNA-Cas9 plasmid concentration requires adjustment to 1 μg μl^-1.
• All relevant table concentrations should also be updated to μg μl^-1.
• These corrections are vital for the successful execution of the described experimental methods.

Impact:

• Improved accuracy and reproducibility of experiments using this protocol.
• Prevents potential experimental failures due to incorrect reagent concentrations.
• Enhances the reliability of scientific data derived from this methodology.