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Morin Protects Channel Catfish From Aeromonas hydrophila Infection by Blocking Aerolysin Activity

Jing Dong1,2, Yongtao Liu1,2, Ning Xu1,2

  • 1Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, China.

Frontiers in Microbiology
|December 7, 2018
PubMed

Insights

Morin, a flavonoid, inhibits Aeromonas hydrophila

Area of Science:

  • Microbiology
  • Biochemistry
  • Pharmacology

Background:

  • Aeromonas hydrophila (A. hydrophila) is an opportunistic pathogen causing infections in humans and animals.
  • Antibiotic resistance necessitates novel therapeutic strategies against A. hydrophila.
  • Aerolysin, a key virulence factor, is a potential drug target.

Purpose of the Study:

  • To investigate the inhibitory effect of morin on Aeromonas hydrophila aerolysin.
  • To elucidate the mechanism of morin's action against aerolysin.
  • To evaluate morin's efficacy in protecting against A. hydrophila infection.

Main Methods:

  • Biochemical assays to assess aerolysin hemolytic activity inhibition.
  • Site-directed mutagenesis and binding assays to identify morin's interaction sites on AerA.
  • Cell culture models (Vero cells) and in vivo studies (channel catfish) to evaluate protection.

Main Results:

  • Morin directly inhibited the hemolytic activity of aerolysin.
  • Morin binds to Ser36, Pro347, and Arg356 residues of AerA, disrupting its conformation and heptamer formation.
  • Morin protected Vero cells from aerolysin-induced damage and provided protection to channel catfish against A. hydrophila infection.

Conclusions:

  • Morin effectively inhibits Aeromonas hydrophila aerolysin activity.
  • Morin's mechanism involves binding to specific residues, preventing aerolysin oligomerization.
  • Morin demonstrates therapeutic potential for treating A. hydrophila infections by reducing its pathogenicity.
Keywords:
Aeromonas hydrophilaaerolysinanti-virulenceantibioticsmorin

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