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Measuring Spray Droplet Size from Agricultural Nozzles Using Laser Diffraction
Published on: September 16, 2016
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Identifying critical process steps to protein stability during spray drying using a vibrating mesh or a two-fluid
Niels Grasmeijer1, Valeria Tiraboschi1, Herman J Woerdenbag1
1Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands.
Summary
Choosing the right spray drying nozzle is crucial for protein stability. Two-fluid nozzles better preserve protein activity compared to vibrating mesh nozzles, highlighting process optimization
Area of Science:
- Biotechnology
- Protein Chemistry
- Process Engineering
Background:
- Protein stability during spray drying is critical for biopharmaceutical formulation.
- Different nozzle types can significantly impact protein integrity.
Purpose of the Study:
- To identify critical steps affecting protein stability during spray drying.
- To compare protein stability using a vibrating mesh nozzle versus a two-fluid nozzle.
Main Methods:
- Spray drying of l-Lactic dehydrogenase with trehalose using two nozzle types.
- Assessing protein enzymatic activity after each spray drying step.
- Analyzing contributions of atomization, heating, dehydration, and circulation to activity loss.
Main Results:
- Vibrating mesh nozzle resulted in 78% activity loss, primarily from atomization and heating.
- Two-fluid nozzle showed only 23% activity loss, with atomization, dehydration, and circulation contributing equally.
- Two-fluid nozzle's cooling capability mitigated heat-induced protein denaturation.
Conclusions:
- Nozzle type and configuration are key factors in maintaining protein stability during spray drying.
- Process optimization, alongside formulation, is essential for preserving protein activity.
- Two-fluid nozzles offer an advantage for protein spray drying due to integrated cooling.
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