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Related Experiment Video

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A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
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Clever Cooperation: Interactions Between EspF and Host Proteins.

Ying Hua1,2, Kaina Yan1,2, Chengsong Wan1,2

  • 1Department of Microbiology, School of Public Health, Southern Medical University, Guangzhou, China.

Frontiers in Microbiology
|December 8, 2018
PubMed
Summary

Enterohemorrhagic Escherichia coli (EHEC) EspF protein interacts with host proteins to promote bacterial colonization and pathogenesis. This review details EspF

Keywords:
EHEC (enterohaemorrhagic E. coli)EPECEspFbacterial pathogenesisprotein interactions

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Area of Science:

  • Microbiology and Molecular Pathogenesis
  • Host-Pathogen Interactions

Background:

  • EspF is a key effector protein secreted by pathogenic Escherichia coli (EHEC, EPEC) and Citrobacter rodentium (CR) via the type III secretion system.
  • Understanding EspF's interactions with host proteins is crucial for elucidating the pathogenesis of these enteric bacterial infections.

Purpose of the Study:

  • To review the functions of the EspF protein.
  • To detail the interactions between EspF and specific host proteins.
  • To explain how these interactions contribute to the pathogenicity of EHEC and EPEC.

Main Methods:

  • Literature review of studies investigating EspF protein function and host interactions.
  • Analysis of reported binding partners of EspF, including SNX9, N-WASP, cytokeratin 18, actin, 14-3-3ζ, Arp2/3, profilin, ZO-1, Abcf2, and Anxa6.
  • Synthesis of information on the cellular processes affected by EspF-host protein complexes.

Main Results:

  • EspF interacts with SNX9 and N-WASP to enhance bacterial colonization of intestinal epithelial cells.
  • EspF modulates host cell cytoskeleton and tight junctions by interacting with cytokeratin 18, actin, 14-3-3ζ, Arp2/3, profilin, and ZO-1.
  • EspF promotes apoptosis via Abcf2 interaction and inhibits phagocytosis through Anxa6 interaction, contributing to EHEC/EPEC virulence.

Conclusions:

  • EspF employs diverse interactions with host proteins to manipulate cellular functions, including cytoskeleton organization, tight junction integrity, apoptosis, and phagocytosis.
  • These multifaceted interactions are central to the pathogenic mechanisms of EHEC and EPEC, highlighting EspF as a critical virulence factor.