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Updated: Feb 1, 2026

Orthotopic Mouse Model of Colorectal Cancer
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Construction and analysis of dysregulated lncRNA-associated ceRNA network in colorectal cancer

Yiping Zhu1,2, Yinzhu Bian3, Qun Zhang4

  • 1Comprehensive Cancer Center, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing, Jiangsu, China.

Journal of Cellular Biochemistry
|December 8, 2018
PubMed

Insights

This study reveals long noncoding RNA LINC00365 as an oncogene in colorectal cancer (CRC). It influences CRC progression by interacting with microRNAs (miRNAs) and messenger RNAs (mRNAs).

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • Colorectal cancer (CRC) is a prevalent digestive system malignancy.
  • Understanding the complex molecular interactions in CRC is crucial for revealing disease mechanisms.

Purpose of the Study:

  • To investigate the intricate interactions among messenger RNAs (mRNAs), microRNAs (miRNAs), and long noncoding RNAs (lncRNAs) in colorectal cancer.
  • To identify key regulatory molecules involved in CRC pathogenesis.

Main Methods:

  • Differential gene expression analysis of public datasets to identify dysregulated mRNAs.
  • Coding-noncoding network analysis to pinpoint regulatory lncRNAs.
  • Validation of gene expression in clinical samples and cell lines.
  • Construction and analysis of a competing endogenous RNA (ceRNA) network.

Main Results:

  • Identified 1,081 common dysregulated mRNAs, suggesting their significant roles in CRC.
  • LINC00365 was identified as a significantly upregulated long noncoding RNA in CRC tissues and cell lines.
  • A ceRNA network involving LINC00365, 70 miRNAs, and 9 mRNAs was constructed.
  • Eight of the nine mRNAs in the network were validated as upregulated in The Cancer Genome Atlas (TCGA) dataset.

Conclusions:

  • LINC00365 functions as an oncogene in colorectal cancer.
  • LINC00365 may regulate multiple mRNA targets by sponging specific miRNAs, contributing to CRC development.
Keywords:
bioinformatics analysiscolorectal cancercompeting endogenous RNAlong noncoding RNAweighted gene corepression analysis

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