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Point-of-Care Tissue Analysis Using Miniature Mass Spectrometer
Ran Zou1,2, Wenbo Cao1, Leelyn Chong2,3
1State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument , Tsinghua University , Beijing 100084 , China.
Analytical Chemistry
|December 12, 2018
Summary
Direct tissue sampling with miniature mass spectrometry enables rapid point-of-care analysis. This method profiles lipids and identifies disease biomarkers like 2-hydroxylglutarate in clinical samples.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Clinical Diagnostics
Background:
- Point-of-care diagnostics require rapid and accurate analytical techniques.
- Miniature mass spectrometry offers potential for portable clinical analysis.
- Direct tissue sampling methods are needed for simplified sample preparation.
Purpose of the Study:
- To develop and validate a miniature mass spectrometry system for direct tissue analysis.
- To profile lipid species and identify disease-specific biomarkers in tissue samples.
- To demonstrate the capability for rapid lipid identification and relative quantitation.
Main Methods:
- Utilized direct tissue sampling with extraction spray ionization.
- Employed a home-built miniature mass spectrometer (Mini 12).
- Incorporated photochemical (Paternò-Büchi) reactions for lipid C═C bond location identification.
Main Results:
- Successfully profiled lipid species in rat brain, kidney, and liver tissues within minutes.
- Achieved fast identification of lipid C═C locations.
- Quantified relative changes in lipid C═C isomers (e.g., FA 18:1 (Δ9)/FA 18:1 (Δ11)) in mouse cancerous breast tissue.
- Identified accumulation of 2-hydroxylglutarate in human glioma samples.
Conclusions:
- Miniature mass spectrometry with direct sampling is a viable approach for point-of-care clinical applications.
- The system enables rapid lipid profiling and disease biomarker detection.
- This technology facilitates quick diagnosis through identification of specific metabolites like 2-hydroxylglutarate.
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