Related Experiment Video
Updated: Feb 1, 2026

A Novel Model of Mild Traumatic Brain Injury for Juvenile Rats
Published on: December 8, 2014
Juvenile rat and pediatric trazodone studies: how to gain extra sensitivity to overcome bioanalytical challenges
Silvana Olivieri1, Graziella Bovi1, Vanessa Petrucci1
1Angelini RR&D (Research, Regulatory & Development), Angelini SpA, Piazzale della Stazione snc, 00071 - S Palomba-Pomezia (Rome), Italy.
Aim:
Trazodone (TZD) is used for the treatment of depression in adults and, off-label, as a sleep medication in adult and pediatric populations. The off-label use is well documented, however further clinical studies are needed to confirm its efficacy and safety for the treatment of sleep disorders. In this scenario, we developed a bioanalytical method to quantify low TZD concentrations in samples collected by capillary microsampling (CMS) to support dose finding, Good Laboratory Practice juvenile rat toxicokinetic and upcoming pediatric studies.
Methodology:
A method using only 8 μl of plasma was developed and successfully used for analyzing CMS samples from juvenile rats throughout toxicokinetic study.
Conclusion:
By harmoniously maximizing each analytical step, we achieved a sensitive method to quantify TZD in CMS samples.
More Related Videos
Related Concept Videos
Gain
Gain:
Suppose Vin is the input and Vout is the output signal to a circuit.
Sputum Studies II: Culture and Sensitivity
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
Urine Studies II: Urine Culture and Sensitivity Test
Bioavailability Enhancement: Determination and Conceptual Approaches in Overcoming Bioavailability Problems
Pharmacokinetics in Pediatric Patients: Drug Excretion
Pharmacokinetics in Pediatric Patients: Drug Distribution

