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VIP modulates the ALX/FPR2 receptor axis toward inflammation resolution in a mouse model of bacterial keratitis

Thomas W Carion1, David Kracht1, Eliisa Strand1

  • 1Department of Ophthalmology, Visual & Anatomical Sciences, Wayne State University School of Medicine, Detroit, MI 48201, United States.

Prostaglandins & Other Lipid Mediators
|December 12, 2018
PubMed

Insights

Vasoactive intestinal peptide (VIP) treatment effectively reduces bacterial keratitis severity in mice. This peptide influences the formyl peptide receptor 2 (FPR2) pathway, offering a potential therapeutic strategy for eye infections.

Area of Science:

  • Ophthalmology
  • Immunology
  • Pharmacology

Background:

  • Corneal inflammation is a significant cause of vision impairment.
  • Vasoactive intestinal peptide (VIP) is known to modulate inflammatory processes.
  • Formyl peptide receptor 2 (FPR2) is implicated in inflammation resolution.

Purpose of the Study:

  • To investigate the therapeutic potential of VIP in bacterial keratitis.
  • To elucidate the role of the VIP-FPR2 axis in disease outcome.
  • To explore VIP's mechanism of action in resolving inflammation.

Main Methods:

  • Bacterial keratitis was induced in C57BL/6 mice using Pseudomonas aeruginosa.
  • Mice received topical VIP or PBS treatment post-infection.
  • Clinical scores, bacterial load, and inflammatory markers (Griess, MPO) were assessed.

Main Results:

  • Topical VIP treatment significantly reduced clinical scores and bacterial counts.
  • VIP treatment effectively abrogated the inflammatory disease response.
  • VIP's effects on FPR2 pathway activation were observed independently of canonical VIP receptors.

Conclusions:

  • VIP demonstrates therapeutic efficacy in a mouse model of bacterial keratitis.
  • VIP influences the FPR2 pathway, suggesting a novel immunoresolving mechanism.
  • Targeting the VIP-FPR2 axis presents a potential therapeutic intervention for bacterial keratitis.
Keywords:
InflammationLipid mediatorsMouseOcular infectionResolution

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