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Quantifying single-cell secretion in real time using resonant hyperspectral imaging.

José Juan-Colás1,2, Ian S Hitchcock3, Mark Coles4

  • 1Department of Physics, University of York, Heslington, YO10 5DD York, United Kingdom; jose.juancolas@york.ac.uk.

Proceedings of the National Academy of Sciences of the United States of America
|December 12, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces a label-free, high-throughput method to monitor single-cell protein secretion in real-time. This technique quantifies protein release heterogeneity, aiding in the understanding of physiological disorders.

Keywords:
label-freephotonic biosensingphotonic crystalsingle-cell analysis

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Biophysics

Background:

  • Cell communication relies on secreted proteins, and abnormal secretion patterns often signal disease.
  • Monitoring individual cell protein secretion kinetics is vital for understanding systemic malfunctions.

Purpose of the Study:

  • To develop a label-free, high-throughput method for real-time, single-cell protein secretion analysis.
  • To demonstrate the application of this technology in quantifying protein secretion heterogeneity.

Main Methods:

  • Utilized hyperspectral photonic crystal resonant technology for label-free, high-throughput analysis.
  • Performed parallel, in vitro, real-time monitoring of specific single-cell signaling.
  • Quantified thrombopoietin expression heterogeneity in HepG2 liver cells.

Main Results:

  • Successfully demonstrated real-time, single-cell protein secretion analysis.
  • Quantified heterogeneity in thrombopoietin secretion from individual HepG2 cells in response to platelet desialylation.
  • Showcased the method's ability to map real-time protein secretion dynamics.

Conclusions:

  • Mapping real-time protein secretion at single-cell resolution offers a powerful approach for studying complex physiological systems.
  • The developed technology provides a simple yet effective tool for analyzing protein production regulation.
  • This method can aid in unraveling systemic malfunctions indicated by inhomogeneous protein secretion.