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Updated: Feb 1, 2026

Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling
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Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling

Published on: April 2, 2012

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Codon-Optimization of Wild-Type Adeno-Associated Virus Capsid Sequences Enhances DNA Family Shuffling while

Marti Cabanes-Creus1,2, Samantha L Ginn3, Anais K Amaya3

  • 1Translational Vectorology Group, Children's Medical Research Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.

Molecular Therapy. Methods & Clinical Development
|December 12, 2018
PubMed
Summary

A new codon optimization algorithm enhances adeno-associated virus (AAV) capsid engineering by increasing sequence identity. This improves the efficiency of creating diverse AAV variants for gene therapy applications.

Keywords:
AAVDNA shufflingcapsidcodon optimizationdirected evolutionlibrary

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