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Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging
Published on: July 1, 2021
Unsuspected reduced radiochemical purity of the 18F-FDG may decrease image resolution, SUV reliability and diagnostic
Ssang Tae Kim1, Chul Soon Yong, Eun Ok Han
1Cyclotron Center Department of Nuclear Medicine, CareCamp Inc, Daegu 42415, Korea. haneunok@gmail.com.
Objective:
Our aim was to identify the conditions required to stably maintain the radiochemical purity of fluorine-18-fluorodeoxyglucose (18F-FDG) above the Korean Pharmacology (KP) and United States Pharmacology (USP) standards for expiration time (time from the end of synthesis (EOS), 8h even at a high radioactive concentration exceeding 7.4GBq/mL.
Subjects And Methods:
The changes in the radiochemical purity of 18F-FDG were assessed according to the changes in radioactive concentration, ethanol (EtOH) concentration, amount of water for dilution storage temperature, and storage volume.
Results:
Controlling the radioactive concentration as much as possible during the production of 18F-FDG is necessary to improve the radiochemical purity of 18F-FDG. In the production of 18F-FDG, a radioactive concentration <7.4GBq/mL was sufficient to maintain the radiochemical purity above the KP and USP standards for 10h after the EOS. If the radioactive concentration exceeded 7.4GBq/mL during synthesis, the addition of EtOH to 18F-FDG is essential to maintain the radiochemical purity above the KP and USP standards. To minimize residual solvent EtOH production, the addition of 0.1% EtOH to the 18F-FDG is the ideal combination.
Conclusion:
Increasing the radiochemical purity of the 18F-FDG increases the quality of images, the reliabity of the SUV during PET scanning and consequently increases the accuracy of diagnosis. Furthermore, 18F-FDG can be synthesized at a high radioactive concentration in large volume, and its effective date could also be prolonged.
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