Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Genetic Engineering?00:49

What is Genetic Engineering?

80.2K
Overview
80.2K
DNA-only Transposons02:57

DNA-only Transposons

17.4K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
17.4K
Overview of DNA Repair02:25

Overview of DNA Repair

33.7K
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
33.7K
DNA Topoisomerases02:02

DNA Topoisomerases

35.5K
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
35.5K
DNA Helicases00:55

DNA Helicases

24.1K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
24.1K
Recombinant DNA01:09

Recombinant DNA

102.8K
Overview
102.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tunable Lipid Coatings Enable Cytoplasmic siRNA Delivery by DNA Origami.

ACS applied materials & interfaces·2026
Same author

Hierarchy of Hydrophobic and Electrostatic Interactions in DNA-Membrane Phase Selectivity.

ACS applied materials & interfaces·2025
Same author

Pattern and precision: DNA-based mapping of spatial rules for T cell activation.

Nanoscale horizons·2025
Same author

Multivalent engineering of bio interfaces with DNA-based nanomaterials.

Advanced drug delivery reviews·2025
Same author

Stimuli-Responsive Oligolysine-PEG Coatings for Reductive-Triggered Decomplexation.

ACS polymers Au·2025
Same author

Evolution of multivalent supramolecular assemblies of aptamers with target-defined spatial organization.

Nature nanotechnology·2025

Related Experiment Video

Updated: Feb 1, 2026

Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation
12:03

Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation

Published on: July 23, 2015

15.0K

Engineering a stable future for DNA-origami as a biomaterial.

Hale Bila1, Eva E Kurisinkal, Maartje M C Bastings

  • 1Programmable Biomaterials Laboratory (PBL), Institute of Materials (IMX)/Interfaculty Bioengineering Institute (IBI), School of Engineering (STI), École Polytechnique Federale de Lausanne (EPFL), MXC 340, Station 12, CH-1015, Lausanne, Switzerland. Maartje.Bastings@epfl.ch.

Biomaterials Science
|December 12, 2018
PubMed
Summary

DNA origami biomaterials offer exciting potential for medicine but require high cation concentrations for stability. This review compares methods to overcome challenges like low cation levels and nucleases, enabling broader bioengineering applications.

More Related Videos

Designing a Bio-responsive Robot from DNA Origami
13:32

Designing a Bio-responsive Robot from DNA Origami

Published on: July 8, 2013

22.8K
Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
08:30

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

9.7K

Related Experiment Videos

Last Updated: Feb 1, 2026

Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation
12:03

Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation

Published on: July 23, 2015

15.0K
Designing a Bio-responsive Robot from DNA Origami
13:32

Designing a Bio-responsive Robot from DNA Origami

Published on: July 8, 2013

22.8K
Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
08:30

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

9.7K

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Molecular Engineering

Background:

  • DNA is a promising biomaterial for therapeutics, diagnostics, and hydrogel scaffolds due to its programmable shape and functionality.
  • Current DNA-based materials face stability challenges in physiological conditions, including low cation concentrations and nuclease degradation.
  • Ensuring the integrity of DNA materials is crucial for their translation into practical bioengineering applications.

Purpose of the Study:

  • To review common stability issues associated with DNA origami biomaterials.
  • To compare various recently developed strategies for enhancing DNA origami stability.
  • To provide a guide for selecting appropriate stabilization methods for future users.

Main Methods:

  • Literature review of DNA origami stability challenges.
  • Analysis and comparison of different DNA origami stabilization techniques.
  • Overview of methods addressing cation concentration and nuclease degradation.

Main Results:

  • Identified high cation concentration requirement for DNA self-assembly and denaturation prevention.
  • Highlighted the threat of low cation levels and nucleases in physiological and cell-culture environments.
  • Detailed various stabilization strategies developed to overcome these weaknesses.

Conclusions:

  • DNA origami technology requires robust stabilization for successful bioengineering translation.
  • A comparative overview of stabilization methods is presented to aid researchers in method selection.
  • Addressing stability issues is key to unlocking the full potential of DNA origami as a biomaterial.