Related Experiment Video
Updated: Feb 1, 2026
Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
Published on: January 9, 2019
Histological and immunohistochemical study on the apical granuloma
Alexandra Carina Bănică1, Sanda Mihaela Popescu, Veronica Mercuţ
1Research Center for Microscopic Morphology and Immunology, University of Medicine and Pharmacy of Craiova, Romania; dr_cristinab@yahoo.com.
Introduction:
Periapical granuloma is one of the most frequent periodontal pathology and belongs to the group named as apical periodontitis.
Materials And Methods:
Out of 78 of diagnosed granulomas, we selected samples that we analyzed histologically and immunohistochemically.
Results:
The histopathological aspect has been dominated by the presence of mononuclear cells of the lymphocyte and plasma cells type, showing the chronic aspect of the apical lesion. Also, we noticed that in the apical granuloma macrophages occur most often. This density of macrophages explains cellular and tissular disruption that occur in apical region of the tooth under the influence of bacterial flora that reached this area, as they have the role to phagocyte pathogens and cell and tissue residues that result from bacterial aggression. The reaction of the plasma cells, determined by their number, has been always associated with the age of the granulomas, and it is more intense in old, neglected granulomas, compared to recent granulomas.
Conclusions:
The number and type of immunity cells varies in the apical granuloma accordingly to the age of granuloma.
Related Concept Videos
01:17Assessment of apical pulse
Assessing the apical pulse is a critical nursing procedure, particularly indicated for:
01:25Assessment of apical radial pulse
The A-R pulse assessment involves simultaneous evaluation of the apical and radial pulses. When the apical and radial pulse rates vary, this assessment helps identify a pulse deficit.
Pre-Procedural Preparation
01:26Stomach Histology
01:27Liver Histology
Hepatocytes perform a variety of essential functions. They secrete...
01:26Histology of the Large Intestine
The innermost mucosa layer comprises simple columnar epithelium, lamina propria, and muscularis mucosae. This layer is primarily populated with absorptive cells, tasked with water absorption, and goblet cells, responsible for secreting mucus to...
01:27Histology of the Small Intestine
The intestinal lining features transverse folds called circular folds, each housing fingerlike projections known as intestinal villi. These villi are covered by a layer of simple columnar epithelium, also referred to as...