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Updated: Feb 1, 2026

Sampling, Identification and Characterization of Microplastics Release from Polypropylene Baby Feeding Bottle during Daily Use
Published on: July 24, 2021
Testing baby bottles for the presence of residual and migrated bisphenol A
Manal Ali1, Madi Jaghbir1, Mahmoud Salam2
1Department of Family and Community Medicine, Faculty of Medicine, University of Jordan, Amman, Jordan.
Insights
Bisphenol A (BPA) leaches from polycarbonate baby bottles, with 100% releasing BPA after stress tests. While not exceeding safe limits, prolonged use with other plastics may pose risks.
Area of Science:
- Environmental Science
- Toxicology
- Materials Science
Background:
- Bisphenol A (BPA) is a chemical linked to trans-generational genetic and behavioral changes, obesity, and thyroid dysfunction.
- Polycarbonate (PC) plastics, often used in baby bottles, may contain or release BPA.
- Understanding BPA leaching from baby bottles is crucial for infant health and safety.
Purpose of the Study:
- To detect the presence and quantify the levels of BPA in polycarbonate baby bottles.
- To assess BPA migration under various stress conditions.
- To estimate potential BPA intake in infants and compare it to the tolerable daily intake (TDI).
Main Methods:
- Random selection of fifteen polycarbonate baby bottles marketed as BPA-free.
- High-Performance Liquid Chromatography (HPLC) analysis to detect residual and migrating BPA.
- Three sequential stress tests were applied to simulate usage and washing.
Main Results:
- 27% of bottles initially contained detectable residual BPA.
- 100% of bottles released migrating BPA after the second and third stress tests.
- A significant positive linear trend in BPA levels was observed across stress tests (P < 0.0001), with stress accounting for 73.5% of variability (P < 0.0001).
Conclusions:
- Polycarbonate baby bottles, even those labeled BPA-free, can release BPA, particularly after repeated stress.
- Infant BPA intake from bottles can range from 0.8% to 23.8% of TDI, with combined exposure potentially exceeding limits.
- Recommendations include cautious use of plastic bottles, awareness of usage duration, and manufacturer transparency regarding BPA content and usage guidelines.
Abstract:
Plastic made with bisphenol A (BPA) produces trans-generational changes in genes and behavior. It has been positively linked to obesity and thyroid dysfunction. This study aimed to detect the presence of BPA in polycarbonate plastic (PC) baby bottles. Fifteen PC baby bottles with a clear indication of BPA free/safe/clear were randomly selected. High-performance liquid chromatography (HPLC) tests were used to detect residual or migrating BPA post three stress tests. An estimated intake of BPA among children was calculated then compared to the universal tolerable daily intake (TDI). Around 27% of bottles had detectable amounts of residual BPA in the first test, 100% released migrating BPA in the second and third tests. A significant positive linear trend in migrated BPA levels was observed over the three consecutive tests P < 0.0001. Approximately 73.5% of the accounted variability in BPA levels was due to these stress tests P < 0.0001. Babies from 0 to 3 months are expected to consume 0.8 to 23.8% of their safe TDI of BPA just by using plastic bottles between the first time of usage and after 60 washes (estimated 15 to 20 days of usage). Although no bottles have shown a risk of BPA intake exceeding TDI, the combined use of BPA bottles with other plastic utensils might result in reaching it. It is advisable to refrain from using BPA-containing plastic bottles or be cautious about usage duration. Manufacturers should indicate a clear margin of usage duration. Four baby bottle brands showed the least BPA leaking (Baby King, Camera, Sweet Baby, and Farlin).
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