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Solvent stable microbial lipases: current understanding and biotechnological applications
Priyanka Priyanka1, Yeqi Tan1, Gemma K Kinsella1
1Dublin Institute of Technology, Dublin 1, Ireland.
Biotechnology Letters
|December 12, 2018
Summary
Microbial lipases can be engineered for enhanced stability in organic solvents. Strategies like improving interactions and altering surface charge create versatile biocatalysts for industrial applications.
Area of Science:
- Biotechnology
- Enzyme Engineering
- Biocatalysis
Background:
- Microbial lipases are crucial industrial enzymes.
- Their application in non-aqueous environments is limited by solvent instability.
- Understanding molecular mechanisms of solvent tolerance is essential.
Purpose of the Study:
- To review current knowledge on microbial lipase solvent tolerance.
- To focus on molecular strategies for enhancing lipase stability in non-aqueous media.
Main Methods:
- Literature review of solvent-tolerant lipases.
- Analysis of molecular strategies for improving solvent stability.
- Examination of enzyme stabilization techniques.
Main Results:
- Overview of known solvent-tolerant lipases.
- Strategies include enhancing stabilizing interactions, modifying residue flexibility, and surface charge alteration.
- Judicious selection and stabilization of lipases broaden their application spectrum.
Conclusions:
- Organic solvent-stable lipases are adaptable biocatalysts.
- They are increasingly employed in the food, pharmaceutical, and green manufacturing industries.
- Continued development will expand their role in industrial biocatalysis.
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