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Updated: Feb 1, 2026

Measuring Deformability and Red Cell Heterogeneity in Blood by Ektacytometry
Published on: January 12, 2018
Cryopreservation of Red Blood Cells
Noha A S Al-Otaibi1,2, Nigel K H Slater1, Hassan Rahmoune3
1Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.
Abstract:
Glycerol and trehalose are widely used protective agents in the cryopreservation of red blood cells (RBCs). This chapter presents a protocol for use of these agents as cryoprotectants of RBCs, with post-thaw assessment of cell survival and cellular oxidative-reductive status. The main aim is to provide a framework for further studies aimed at improving RBC survival and function and to supply improved biomaterials for studies on RBC biochemistry, major operations, as well as those for urgent use in emergency room situations.
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