Mesophyll conductance limitation of photosynthesis in poplar under elevated ozone

Finite mesophyll conductance (g_m) reduces the rate of CO₂ diffusion from the leaf intercellular space to the chloroplast and constitutes a major limitation of photosynthesis in trees. While it is well established that g_m is decreased by stressors such as drought and high temperature, few studies have investigated if the phytotoxic air pollutant ozone (O₃) affects g_m. We quantified the relative importance of three different types of limitations of photosynthesis in poplar trees exposed to elevated O₃: decreases in stomatal conductance, g_m and biochemical photosynthetic capacity. The O₃-induced reductions in light-saturated net photosynthesis were linked to significant declines in g_m and biochemical photosynthetic capacity (in particular carboxylation). There was no significant effect of O₃ on stomatal conductance. Of the O₃-induced limitations on photosynthesis, g_m limitation was by far the most important (-16%) while biochemical limitation (-8%) was rather small. Both limitations grew in magnitude over the study period and varied in response to leaf-specific O₃ exposure. Our findings suggest that declines in g_m may play a key role in limiting photosynthesis of plants exposed to elevated O₃, an effect hitherto overlooked.