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Updated: Feb 1, 2026
16:03
A Fish-feeding Laboratory Bioassay to Assess the Antipredatory Activity of Secondary Metabolites from the Tissues of Marine Organisms
Published on: January 11, 2015
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A new multistep purification method for simultaneously determining organic ultraviolet absorbents in fish tissue
Fuyong Zhong1,2, Xianhui Zhang3, Guanghui Li4
1College of Life and Environmental Sciences, Minzu University of China, Beijing, 100081, China.
Environmental Monitoring and Assessment
|December 13, 2018
Summary
This study developed a new purification method to detect organic ultraviolet absorbents (UVAs) in fish tissue. The optimized method accurately measures trace UVA levels, aiding environmental risk assessments.
Area of Science:
- Environmental Chemistry
- Analytical Chemistry
- Toxicology
Background:
- Organic ultraviolet absorbents (UVAs) are increasingly detected in biota, necessitating better understanding of their presence and ecological risks.
- Lipid removal during sample processing is a significant challenge for accurate UVA determination in biological matrices.
Purpose of the Study:
- To optimize a multistep purification method for analyzing UVAs in fish tissue.
- To develop a reliable method for detecting trace concentrations of multiple UVAs in fish samples.
Main Methods:
- Ultrasonic-assisted extraction followed by Florisil column chromatography.
- Dispersive solid-phase extraction using amine, Florisil, and C18 silica sorbents.
- Gas chromatography-mass spectrometry (GC-MS) for analysis.
Main Results:
- The optimized method achieved mean UVA recovery rates from 65.4% to 118%.
- Method detection limits ranged from 0.20 to 2.50 ng/g dry weight for 12 target UVAs.
- The validated method successfully detected trace UVA concentrations in fish tissue samples.
Conclusions:
- The developed multistep purification method is effective and reliable for analyzing UVAs in fish.
- This method facilitates the detection of trace UVA levels, crucial for assessing risks to aquatic organisms.
- The study successfully analyzed 12 UVAs in fish samples from a local supermarket.
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