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Microtomy: Cutting Formalin-Fixed, Paraffin-Embedded Sections.

Joanne Sy1, Lee-Cyn Ang2

  • 1Department of Anatomical Pathology, Concord Repatriation General Hospital, University of Sydney, Concord, NSW, Australia. drjoannesy@gmail.com.

Methods in Molecular Biology (Clifton, N.J.)
|December 13, 2018
PubMed
Summary
This summary is machine-generated.

Formalin-fixed, paraffin-embedded tissue (FFPE) is crucial for biobanking due to its stability. Proper microtomy techniques are essential for preparing FFPE tissue sections for accurate downstream analyses.

Keywords:
FFPEFormalin-fixedMicrotomyParaffin sections

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Area of Science:

  • Histopathology
  • Biobanking
  • Molecular Diagnostics

Background:

  • Formalin-fixed, paraffin-embedded (FFPE) tissue is a vital biobanking resource, offering stability and ease of storage compared to fresh frozen tissue.
  • FFPE tissues support diverse downstream applications, including histology, immunohistochemistry, in situ hybridization, and molecular/proteomic analyses.
  • Pathologist review of stained FFPE sections is critical for confirming cellularity and suitability for molecular testing.

Purpose of the Study:

  • To detail the microtomy process for preparing FFPE tissue sections for biobanking.
  • To identify and discuss potential challenges encountered during FFPE sectioning.
  • To provide guidance on avoiding common pitfalls in FFPE microtomy.

Main Methods:

  • Description of the rotary microtome technique for cutting FFPE tissue sections.
  • Emphasis on meticulous sectioning to preserve tissue integrity.
  • Inclusion of quality control measures for assessing section adequacy.

Main Results:

  • Successful preparation of FFPE tissue sections using rotary microtomy.
  • Identification of common issues such as tearing, compression, and uneven thickness.
  • Strategies for troubleshooting and optimizing section quality were discussed.

Conclusions:

  • Precise microtomy is fundamental for maximizing the utility of FFPE tissues in biobanking.
  • Understanding and mitigating microtomy pitfalls ensures the integrity of FFPE samples for research and diagnostics.
  • This technique facilitates reliable molecular and proteomic analyses from FFPE specimens.